SUMOylation of nuclear actin

2009/07/27 by Wilma A. Hofmann, Alessandro Arduini, Samantha M. Nicol +4 · 4 citations
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · #Actin #Actin cytoskeleton #Actin remodeling #Actin-binding protein #Biology #Cell #Cell biology #Cell nucleus #Chromatin #Cytoplasm #Cytoskeleton #Gene #Genetics #Nuclear protein #Nuclear transport #RNA Research and Splicing #SUMO protein #Transcription factor #Ubiquitin #Ubiquitin and proteasome pathways #interferon and immune responses

paper · pdf · doi:10.1083/jcb.200905016

openalex publication_date 2009/07/27 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/05

Abstract

Actin, a major component of the cytoplasm, is also abundant in the nucleus. Nuclear actin is involved in a variety of nuclear processes including transcription, chromatin remodeling, and intranuclear transport. Nevertheless, the regulation of nuclear actin by posttranslational modifications has not been investigated. We now show that nuclear actin is modified by SUMO2 and SUMO3 and that computational modeling and site-directed mutagenesis identified K68 and K284 as critical sites for SUMOylating actin. We also present a model for the actin-SUMO complex and show that SUMOylation is required for the nuclear localization of actin.

Citations

• Long-Range Directional Movement of an Interphase Chromosome Site
• Chapter 6 Cell and Molecular Biology of Nuclear Actin
• Actin is part of pre-initiation complexes and is necessary for transcription by RNA polymerase II
• Nucleoplasmic β-actin exists in a dynamic equilibrium between low-mobility polymeric species and rapidly diffusing populations
• Tracking down the different forms of nuclear actin
• Nuclear export of actin: a novel mechanism regulating the subcellular localization of a major cytoskeletal protein
• Exportin 6: a novel nuclear export receptor that is specific for profilin·actin complexes

Cited by

• Active maintenance of nuclear actin by importin 9 supports transcription
• β- and γ-Actins in the nucleus of human melanoma A375 cells
• Actin on DNA—An ancient and dynamic relationship
• Actin [wikipedia]

Related

• Actin from within – how nuclear myosins and actin regulate nuclear architecture and mechanics
• Emerging Properties and Functions of Actin and Actin Filaments Inside the Nucleus
• Distinct In Vivo Dynamics of Vertebrate SUMO Paralogues
• Nuclear actin dynamics – From form to function
• β- and γ-Actins in the nucleus of human melanoma A375 cells