2000/09/15 by Jiyong Zhao, Brian K. Kennedy, Brandon D. Lawrence +4 · 5 citations
Biochemistry, Genetics and Molecular Biology · #Biology #Cell biology #Cell cycle #Cyclin A #Cyclin D1 #Cyclin-dependent kinase 2 #Cyclin-dependent kinase 3 #DNA Repair Mechanisms #Epigenetics and DNA Methylation #Gene #Genetics #Genomics and Chromatin Dynamics #Histone #Histone H2A #Histone methyltransferase #Molecular biology
paper · pdf · doi:10.1101/gad.827700
openalex publication_date 2000/09/15 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
In eukaryotic cells, histone gene expression is one of the major events that mark entry into S phase. While this process is tightly linked to cell cycle position, how it is regulated by the cell cycle machinery is not known. Here we show that NPAT, a substrate of the cyclin E-Cdk2 complex, is associated with human replication-dependent histone gene clusters on both chromosomes 1 and 6 in S phase. We demonstrate that NPAT activates histone gene transcription and that this activation is dependent on the promoter elements (SSCSs) previously proposed to mediate cell cycle-dependent transcription. Cyclin E is also associated with the histone gene loci, and cyclin E-Cdk2 stimulates the NPAT-mediated activation of histone gene transcription. Thus, our results both show that NPAT is involved in a key S phase event and provide a link between the cell cycle machinery and activation of histone gene transcription.