2013/09/20 by Peter Heftberger, Benjamin Kollmitzer, Heftberger, Peter +15
Biochemistry, Genetics and Molecular Biology · Physics and Astronomy · #Biological Physics (physics.bio-ph) #Biomolecules (q-bio.BM) #FOS: Biological sciences #FOS: Physical sciences #Soft Condensed Matter (cond-mat.soft) #cond-mat.soft #physics.bio-ph #q-bio.BM
paper · pdf · doi:10.48550/arxiv.1309.5237
arxiv created 2013/09/20 · arxiv updated 2016/11/25
We adapted the highly successful scattering density profile (SDP) model, which is used to jointly analyze small angle x-ray and neutron scattering data from unilamellar vesicles, for use with data from fully hydrated, liquid crystalline multilamellar vesicles (MLVs). By using a genetic algorithm, this new method is capable of providing high resolution structural information, as well as determining bilayer elastic bending fluctuations from standalone x-ray data. Important structural parameters, such as bilayer thickness and area per lipid were determined for a series of saturated and unsaturated lipids, as well as binary mixtures with cholesterol. Results are in good agreement with previously reported SDP data, which used both neutron and x-ray data. The addition of deuterated and non-deuterated MLV neutron data to the analysis improved lipid backbone information, but did not improve, within experimental uncertainties, the structural information regarding bilayer thickness and area per lipid.