2016/10/26 by Michal Belička, Belička, Michal, Anna Weitzer +3
Biochemistry, Genetics and Molecular Biology · Materials Science · Physics and Astronomy · #FOS: Physical sciences #Force Microscopy Techniques and Applications #Lipid Membrane Structure and Behavior #Soft Condensed Matter (cond-mat.soft) #Supramolecular Self-Assembly in Materials #cond-mat.soft
paper · pdf · doi:10.48550/arxiv.1610.08252
openalex publication_date 2016/10/26 · arxiv created 2016/12/16 · arxiv updated 2016/12/19 · openalex created_date 2022/08/14 · openalex updated_date 2026/07/28
We advanced a previously reported model for the in situ scattering data analysis of coexisting lipid domains in free-floating multilamellar vesicles. Based on the scattering density profile of the individual domains we considered in particular modifications due to positionally anticorrelated domains or the presence of cholesterol. The latter aspect can be easily extended to any other lipophilic molecule. This allowed us to retrieve elastic and structural details (bending fluctuations, domain thickness, area per lipid) of coexisting liquid-ordered/liquid-disordered domains, including the partitioning of cholesterol from small-angle x-ray scattering experiments. Moreover, the model also successfully fitted data of multilamellar vesicles exhibiting nanoscopic domains, which displayed unlike coexisting micron-sized domains no clear signal for two lamellar lattices. Our results indicate a previously not observed translation of cholesterol toward the bilayer center of nanoscopic liquid-ordered domains.