2024/09/29 by Gries, Wolfgang, Küpper, Katja, Leng, Gabriele +6
#(±)-1 #3-(4′-Carboxybenzyliden)-6-hydroxycampher #3-(4′-Carboxybenzyliden)campher #3-(4′-Carboxybenzyliden)hydroxycampher #3-(4′-Carboxybenzylidene)-6-hydroxycamphor #3-(4′-Carboxybenzylidene)camphor #3-(4′-Methylbenzyliden)bornan-2-on #3-(4′-Methylbenzyliden)campher #3-(4′-Methylbenzylidene)bornan-2-one #3-(4′-Methylbenzylidene)camphor #3-(4′-carboxybenzylidene)hydroxycamphor #4-MBC #4-[(4 #4-[(5-Hydroxy-1 #4-[(6-Hydroxy-4 #543 Analytical chemistry #543 Analytische Chemie #571.95 Toxicology #571.95 Toxikologie #616.9803 Betriebs- und Arbeitsmedizin #616.9803 Industrial and occupational medicine #7 #7-Trimethyl-3-[(4-methylphenyl)methylen]bicyclo[2.2.1]heptan-2-on #7-Trimethyl-3-[(4-methylphenyl)methylene]bicyclo[2.2.1]heptan-2-one #7-Trimethyl-3-oxobicyclo[2.2.1]hept-2-yliden)methyl]benzoesäure #7-Trimethyl-3-oxobicyclo[2.2.1]hept-2-ylidene)methyl]benzoic acid #7-trimethyl-2-oxobicyclo[2.2.1]hept-3-yliden)methyl]benzoesäure #7-trimethyl-2-oxobicyclo[2.2.1]hept-3-ylidene)methyl]benzoic acid #7-trimethyl-3-oxobicyclo[2.2.1]hept-2-yliden)methyl]benzoesäure #7-trimethyl-3-oxobicyclo[2.2.1]hept-2-ylidene)methyl]benzoic acid #Allgemeinbevölkerung #Analysen in biologischem Material #Analysenmethode #Analyses in Biological Materials #Arbeiter #Arbeitnehmer #Arbeitsplatz #Arbeitsplatzbelastung #Biomonitoring #Biomonitoringmethode #HBM #Hintergrundbelastung #Humanbiomonitoring #Intoxikation #Methode #UPLC-MS/MS #Ultra-Hochleistungsflüssigkeitschromatographie mit Tandem-Massenspektrometrie #Ultra-high-performance liquid chromatography with tandem mass spectrometry #Umweltbelastung #Urin #Vergiftung #analytical method #background exposure #biological monitoring #biologisches Monitoring #biomonitoring #biomonitoring method #cx-MBC #cx-MBC-OH #employee #environmental exposure #general population #human biomonitoring #intoxication #method #poisoning #urine #worker #workplace #workplace exposure
paper · doi:10.34865/bi3686147e9_3or
The working group “Analyses in Biological Materials” of the German Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area (MAK Commission) developed and verified the presented biomonitoring method. The aim of this method is the selective and sensitive quantitation of the two main metabolites of the UVB filter 3‑(4′‑methylbenzylidene)camphor (4‑MBC), 3‑(4′‑carboxybenzylidene)camphor (cx‑MBC) and 3‑(4′‑carboxybenzylidene)hydroxycamphor (cx‑MBC‑OH), in urine. After adding deuterated internal standards (cx‑MBC‑d4 and cx‑MBC‑OH‑d4), the samples are enzymatically hydrolysed. After extraction and enrichment by online SPE, the analytes are separated by liquid chromatography and analysed by tandem mass spectrometry. Good precision data with standard deviations below 5.5% for cx‑MBC and below 6.5% for cx‑MBC‑OH, as well as good accuracy data with mean relative recoveries in the range of 90–110% for cx‑MBC and 89–106% for cx‑MBC‑OH, show that the method provides reliable and accurate analytical results. The method is both selective and sensitive, and the limits of quantitation of 0.15 μg/l (cx‑MBC) and 0.3 μg/l (cx‑MBH‑OH) are sufficient to determine background exposure in the general population.