2023/12/19 by Scherer, Gerhard, Hagedorn, Heinz-Werner, Hoppe, Hans-Wolfgang +5
#3 #4-Benzopyren #4-Benzopyrene #4-Benzpyren #4-Benzpyrene #543 Analytical chemistry #543 Analytische Chemie #571.95 Toxicology #571.95 Toxikologie #616.9803 Betriebs- und Arbeitsmedizin #616.9803 Industrial and occupational medicine #Allgemeinbevölkerung #Analysen in biologischem Material #Analysenmethode #Analyses in Biological Materials #Arbeiter #Arbeitnehmer #Arbeitsplatz #Arbeitsplatzbelastung #Benz[a]pyren #Benz[a]pyrene #Benzo[a]pyren #Benzo[a]pyrene #Benzo[d #Biomonitoring #Biomonitoringmethode #Flüssigkeitschromatographie mit Tandem-Massenspektrometrie #HBM #Hintergrundbelastung #Humanbiomonitoring #Intoxikation #LC-MS/MS #Liquid chromatography with tandem mass spectrometry #Methode #Umweltbelastung #Urin #Vergiftung #analytical method #background exposure #biological monitoring #biologisches Monitoring #biomonitoring #biomonitoring method #e #employee #environmental exposure #f]chrysen #f]chrysene #general population #human biomonitoring #intoxication #method #poisoning #urine #worker #workplace #workplace exposure
paper · doi:10.34865/bi13345e8_4or
The working group “Analyses in Biological Materials” of the German Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and verified the presented biomonitoring method. Benzo[a]pyrene (B[a]P) is a polycyclic aromatic hydrocarbon and is classified as carcinogenic to humans by the Commission. B[a]P originates, for example, from coal tar or from the incomplete combustion of organic material. The general population is primarily exposed through cigarette smoke, food, and ambient air. The aim of this work was to develop a selective method for the determination of 3‑hydroxybenzo[a]pyrene (3‑OH‑B[a]P) in urine. The method is characterised by the use of glucuronidated standards, ascorbic acid as an antioxidant, and by the conversion of the analyte into a derivative which allows for sensitive measurement. The procedure has been comprehensively validated, and the reliability data have been confirmed by replication and verification of the procedure in a second, independent laboratory. For the determination of 3‑OH‑B[a]P, an internal standard is added to the urine samples, which undergo solid-phase extraction prior to analysis by liquid chromatography‑tandem mass spectrometry. Quantitative evaluation is carried out via external calibration using pooled urine from persons with no known exposure to B[a]P. The good precision and accuracy data show that the method provides reliable and accurate analytical results. The method is both selective and sensitive, and the limit of detection of 17 pg/l urine is sufficient to determine both occupational exposure and exposure to B[a]P in the general population.