2026/01/14 by Ujani Chakraborty, Emma Saccone, GRISEL L CRUZ BECERRA +19 · 1 voice
Biochemistry, Genetics and Molecular Biology · Immunology and Microbiology · #Chromatin #DNA #DNA-binding protein #Genomics and Chromatin Dynamics #Heterologous #Histone #Linker DNA #Nucleosome #Plasma protein binding #Protein–protein interaction #Ubiquitin and proteasome pathways #interferon and immune responses
paper · doi:10.64898/2026.01.14.699602
openalex publication_date 2026/01/14 · openalex created_date 2026/01/16 · openalex updated_date 2026/07/14
ABSTRACT The nucleosome acidic patch is a hub of coordinated engagement by proteins that regulate genomic function. Here we report that S. cerevisiae Dot5 contains an arginine-rich HMGN-like motif that mediates nucleosome acidic patch binding and is required for the cell growth, DNA repair and heterochromatin defects exhibited when the protein is overexpressed. The heterologous expression of camelid single chain antibodies to the nucleosome acidic patch confers a similar range of phenotypes, with the most severe observed when an ‘arginine-anchor’ mode of binding analogous to many endogenous factors is employed. This highlights a delicate balance between nucleosome acidic patch interactors critical for normal cellular functions and dysregulated in disease.