Autophagy Promotes Replication of Influenza A Virus <i>In Vitro</i>

2018/12/10 by Ruifang Wang, Yinxing Zhu, Jiachang Zhao +5 · 1 citation
Medicine · Biochemistry, Genetics and Molecular Biology · #Autophagy in Disease and Therapy #Mosquito-borne diseases and control #Endoplasmic Reticulum Stress and Disease

paper · pdf · doi:10.1128/jvi.01984-18

Abstract

Autophagy impacts the replication cycle of many viruses. However, the role of the autophagy machinery in IAV replication remains unclear. Therefore, we explored the detailed mechanisms utilized by IAV to promote its replication. We demonstrated that IAV NP- and M2-mediated autophagy promotes IAV replication by regulating the AKT-mTOR signaling pathway and HSP90AA1 expression. The interaction of PB2 and HSP90AA1 results in the increase of viral RNA synthesis first; subsequently the binding of NP to LC3 favors vRNP export, and later the interaction of M2 and LC3 leads to an increase in the production of infectious viral particles, thus accelerating viral progeny production. These findings improve our understanding of IAV pathogenicity in host cells.

Citations

• Influenza A Virus Proteins NS1 and Hemagglutinin Along with M2 Are Involved in Stimulation of Autophagy in Infected Cells
• A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS12

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