2025/04/30 by Gries, Wolfgang, Leng, Gabriele, Küpper, Katja +5
#1 #3 #3-Tetramethylbutyl)phenol #4-(1 #4‐tert‐Octylphenol #543 Analytical chemistry #543 Analytische Chemie #571.95 Toxicology #571.95 Toxikologie #616.9803 Betriebs- und Arbeitsmedizin #616.9803 Industrial and occupational medicine #Alkylphenole #Allgemeinbevölkerung #Analysen in biologischem Material #Analysenmethode #Analyses in Biological Materials #Arbeiter #Arbeitnehmer #Arbeitsplatz #Arbeitsplatzbelastung #Biomonitoring #Biomonitoringmethode #Flüssigkeitschromatographie mit Tandem-Massenspektrometrie #HBM #Hintergrundbelastung #Humanbiomonitoring #Intoxikation #LC‐MS/MS #Methode #Umweltbelastung #Urin #Vergiftung #alkylphenols #analytical method #background exposure #biological monitoring #biologisches Monitoring #biomonitoring #biomonitoring method #employee #environmental exposure #general population #human biomonitoring #intoxication #liquid chromatography with tandem mass spectrometry #method #online‐SPE #p-Nonylphenol (branched) #p-Nonylphenol (verzweigt) #poisoning #urine #worker #workplace #workplace exposure
paper · doi:10.34865/bi14066d0022_w
The working group „Analyses in Biological Materials“ of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and validated the presented biomonitoring method. The analytical method described hereinafter permits the selective detection of 4‐tert‐octylphenol as well as the sum of various branched p-nonylphenol isomers in urine. After adding the labelled internal standards (13C6‐4‐tert‐octylphenol and 13C6‐p‐nonylphenol), the samples are enzymatically hydrolysed to release the analytes from the conjugated alkylphenols. After online SPE, the analytes are separated by liquid chromatography and analysed using tandem mass spectrometry. A quantitation limit of 2 µg/l each is obtained for the analytes. Calibration standards are prepared in pooled urine and processed in the same way as the samples to be analysed.