2023/12/19 by Jäger, Thomas, Bader, Michael, Kuhlmann, Laura +3
#2-Phenoxyethanol #543 Analytical chemistry #543 Analytische Chemie #571.95 Toxicology #571.95 Toxikologie #616.9803 Betriebs- und Arbeitsmedizin #616.9803 Industrial and occupational medicine #Allgemeinbevölkerung #Analysen in biologischem Material #Analysenmethode #Analyses in Biological Materials #Arbeiter #Arbeitnehmer #Arbeitsplatz #Arbeitsplatzbelastung #Biomonitoring #Biomonitoringmethode #Ethylene glycol monophenyl ether #Ethylenglykolmonophenylether #HBM #Hintergrundbelastung #Humanbiomonitoring #Intoxikation #Methode #UPLC-ESI-MS/MS #Ultra-Hochleistungsflüssigkeitschromatographie mit Elektrospray-Ionisation und Tandem-Massenspektrometrie #Ultra-high-performance liquid chromatography with electrospray ionisation and tandem mass spectrometry #Umweltbelastung #Urin #Vergiftung #analytical method #background exposure #biological monitoring #biologisches Monitoring #biomonitoring #biomonitoring method #employee #environmental exposure #general population #human biomonitoring #intoxication #method #poisoning #urine #worker #workplace #workplace exposure
paper · doi:10.34865/bi12299e8_4or
The working group “Analyses in Biological Materials” of the German Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and verified the presented method for the biomonitoring of exposure to 2‑phenoxyethanol, an important biocide. The method described herein allows for the determination of the two main metabolites of 2‑phenoxyethanol, specifically phenoxyacetic acid (PhAA) and 4‑hydroxyphenoxyacetic acid (4‑OH‑PhAA), in urine. The urine samples are mixed with diluted formic acid and the internal standards. The analytes are separated from matrix components by liquid chromatography and subsequently analysed by tandem mass spectrometry using electrospray ionisation. Quantitative evaluation is carried out via external calibration in ultra‑pure water. Good precision data with standard deviations below 7.19% for PhAA and below 8.07% for 4‑OH‑PhAA as well as good accuracy data with mean relative recoveries in the range of 98.7–109% for PhAA and of 92.6–107% for 4‑OH‑PhAA show that the method provides reliable and accurate analytical results. The method is both selective and sensitive, and the limits of quantitation of 8.2 μg PhAA/l urine and 7.6 μg 4‑OH‑PhAA/l urine are sufficient to determine occupational exposure as well as background exposure of the general population to 2‑phenoxyethanol.