2013/03/25 by Simone Nicolardi, Yuri E. M. van der Burgt, І. В. Драган +2 · 51 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · #Advanced Proteomics Techniques and Applications #Apolipoprotein B #Biochemistry #Chemistry #Cholesterol #Chromatography #Fourier transform ion cyclotron resonance #Fucose #Fucosylation #Gene isoform #Glycan #Glycoprotein #Glycosylation #Glycosylation and Glycoproteins Research #Machine Learning in Bioinformatics #Mass spectrometry #Sialic acid
paper · doi:10.1021/pr400136p
published in Journal of Proteome Research 12(5), 2260-2268 (American Chemical Society)
openalex publication_date 2013/03/25 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/15
Apolipoprotein-CIII (apoCIII) is an abundant blood glycoprotein associated with lipoprotein particles. Three different glycoforms have been described, all containing a mucin-type core-1 O-glycosylation with either zero, one or two sialic acids. Changes in the relative abundance of these glycoforms have been observed in a variety of different pathologies. In this study, ultrahigh resolution 15T MALDI Fourier transform ion cyclotron resonance (FTICR) MS was used to analyze apoCIII isoforms in serum protein profiles. For this purpose, serum proteins were purified using both a fully automated RPC18-based magnetic bead method and an RPC4 cartridge-based solid phase extraction method. Six new apoCIII isoforms were identified with low-ppm mass measurement errors and ultrahigh precision. These were characterized by more complex glycan moieties that are fucosylated instead of sialylated. To confirm the glycan moiety and localize the glycosylation site, top-down ESI-FTICR-MS/MS and bottom-up LC-ion trap MS/MS were used. A large variation in the presence and abundance of the fucosylated isoforms was found in a set of 96 serum samples. These findings of fucosylated apolipoprotein-CIII isoforms warrant further research to elucidate the implications these glycoforms may have for the plethora of studies where alterations in apoCIII have been linked to the development of many different pathologies.