The Complete Amino Acid Sequence of Alanine Apolipoprotein (apoC-III), an Apolipoprotein from Human Plasma Very Low Density Lipoproteins

1974/08/01 by H Bryan Brewer, Richard S. Shulman, Peter N. Herbert +2 · 9 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · #Alanine #Amino acid #Apolipoprotein B #Apolipoprotein C2 #Biochemistry #Chemistry #Cholesterol #Chromatography #Gene #Glycosylation and Glycoproteins Research #Human plasma #Lipoprotein #Machine Learning in Bioinformatics #Peptide sequence #Plasma lipoprotein #Protein Structure and Dynamics #Very low-density lipoprotein

paper · doi:10.1016/s0021-9258(19)42416-2

openalex publication_date 1974/08/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/23

Abstract

Abstract The alanine apolipoprotein (apoLp-Ala) was isolated from patients with familial hyperlipoproteinemia (type V). ApoLp-Ala is a single chain protein of 79 amino acids with a single carbohydrate side chain containing 1 mole each of galactosamine and galactose, and 0 to 2 moles of sialic acid per mole of protein. The complete amino acid sequence of apoLp-Ala was determined by manual and automated Edman degradations of the intact protein, and tryptic and succinylated tryptic peptides. The NH2-terminal residue is serine, the COOH-terminal residue alanine. The carbohydrate moiety is attached to threonine at position 74 in the sequence. The amino acid sequence of apoLp-Ala contains no long sequences of hydrophobic residues which contrasts with the linear amphipathic regions present in the two integral membrane proteins cytochrome b5 and glycophorin.

Citations

• A Protein Sequenator
• Amino Acid Sequence of Human apoLp-Gln-II (apoA-II), an Apolipoprotein Isolated from the High-Density Lipoprotein Complex

Cited by

• Characterization of the oligosaccharide side chain of apolipoprotein C-III from human plasma very low density lipoproteins
• Molecular cloning of a human apoC-III variant: Thr 74----Ala 74 mutation prevents O-glycosylation.
• Isolation and characterization of cDNA clones corresponding to two different human apoC-III alleles.
• Amino acid sequence of human plasma apolipoprotein C‐III from normalipidemic subjects
• Identification of New Apolipoprotein-CIII Glycoforms with Ultrahigh Resolution MALDI-FTICR Mass Spectrometry of Human Sera
• Linkage of human apolipoproteins A-I and C-III genes
• Isolation and Sequence Analysis of the Human Apolipoprotein CIII Gene and the Intergenic Region between the Apo AI and Apo CIII Genes
• Isolation and DNA Sequence of Full-Length cDNA for Human Preapolipoprotein CIII
• Apolipoprotein C-III [wikipedia]