2001/11/01 by Yasuhiro Inoue, Masahiro Miyazaki, Toshiya Tsuji +4 · 1 citation
Medicine · #Pancreatic function and diabetes #Pediatric Hepatobiliary Diseases and Treatments #Pancreatic and Hepatic Oncology Research #Transfection #Hepatocyte nuclear factor 4 #Molecular biology #Hepatocyte #Biology #Hepatocyte nuclear factors #Cell culture #Gene #Liver cell #Cell #Oncogene #Hep G2 #Immortalised cell line #Gene expression #Cell cycle #Transcription factor #Biochemistry #Genetics #In vitro #Internal medicine
paper · doi:10.3892/ijmm.8.5.481
openalex publication_date 2001/11/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/06/11
An immortalized human hepatocyte cell line (OUMS-29) was established from fetal liver by transfection with the SV-40 large T antigen gene that has certain liver-specific functions such as albumin production and enzyme activities of CYP1A1, 1A2, and 2E1. To make OUMS-29 cells express other liver-specific functions, the human hepatocyte nuclear factor 4alpha2 (HNF4alpha2) gene was introduced into the cells, because this gene was found to be markedly down-regulated. The transduced HNF4alpha2 was overexpressed in the nuclei of the transfected cells, and its DNA-binding activity was also detected. The liver-specific genes such as apolipoprotein AI, CII, CIII, blood coagulation factor X, alpha1-antitrypsin, and HNF1alpha were up-regulated. Thus, this cell line is expected to be a useful tool for studying the differentiated human hepatocyte functions.