Evaluation and optimisation of preparative semi‐automated electrophoresis systems for Illumina library preparation

2012/09/07 by Michael A. Quail, Yong Gu, Harold Swerdlow +1 · 2 citations
Environmental Science · Biochemistry, Genetics and Molecular Biology · Computer Science · Chemistry · #Environmental DNA in Biodiversity Studies #Genomics and Phylogenetic Studies #Advanced Data Storage Technologies #Chromatography #Selection (genetic algorithm) #DNA sequencing #Computational biology #Computer science #DNA #Chemistry #Biology #Genetics #Artificial intelligence

paper · doi:10.1002/elps.201200128

openalex publication_date 2012/09/07 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/24

Abstract

Size selection can be a critical step in preparation of next-generation sequencing libraries. Traditional methods employing gel electrophoresis lack reproducibility, are labour intensive, do not scale well and employ hazardous interchelating dyes. In a high-throughput setting, solid-phase reversible immobilisation beads are commonly used for size-selection, but result in quite a broad fragment size range. We have evaluated and optimised the use of two semi-automated preparative DNA electrophoresis systems, the Caliper Labchip XT and the Sage Science Pippin Prep, for size selection of Illumina sequencing libraries.

Citations

• Next-generation sequencing transforms today's biology
• A strategy of DNA sequencing employing computer programs

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• Challenges in the application of NGS in the clinical laboratory
• DNA sequencing [wikipedia]

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