2014/02/01 by Steven R. Head, H. Kiyomi Komori, Sarah LaMere +5 · 3 citations
Biochemistry, Genetics and Molecular Biology · #Base sequence #Biology #Computational biology #Context (archaeology) #DNA #DNA sequencing #Deep sequencing #Gene #Genetics #Genome #Genomic library #Genomics #Genomics and Phylogenetic Studies #Massive parallel sequencing #RNA #RNA and protein synthesis mechanisms #RNA modifications and cancer
paper · doi:10.2144/000114133
openalex publication_date 2014/02/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/05
High-throughput sequencing, also known as next-generation sequencing (NGS), has revolutionized genomic research. In recent years, NGS technology has steadily improved, with costs dropping and the number and range of sequencing applications increasing exponentially. Here, we examine the critical role of sequencing library quality and consider important challenges when preparing NGS libraries from DNA and RNA sources. Factors such as the quantity and physical characteristics of the RNA or DNA source material as well as the desired application (i.e., genome sequencing, targeted sequencing, RNA-seq, ChIP-seq, RIP-seq, and methylation) are addressed in the context of preparing high quality sequencing libraries. In addition, the current methods for preparing NGS libraries from single cells are also discussed.