A strategy of DNA sequencing employing computer programs

1979/01/01 by Rodger Staden · 399 citations
Biochemistry, Genetics and Molecular Biology · #Genomics and Phylogenetic Studies #Genetics, Bioinformatics, and Biomedical Research #RNA and protein synthesis mechanisms #Biology #DNA sequencing #Sequence (biology) #Computational biology #Computer program #DNA #Restriction map #Genome #Whole genome sequencing #Genetics #Nucleic acid sequence #Computer science #Gene #Programming language

paper · open access · doi:10.1093/nar/6.7.2601

published in Nucleic Acids Research 6(7), 2601-2610 (Oxford University Press)

openalex publication_date 1979/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/30

Abstract

With modern fast sequencing techniques and suitable computer programs it is now possible to sequence whole genomes without the need of restriction maps. This paper describes computer programs that can be used to order both sequence gel readings and clones. A method of coding for uncertainties in gel readings is described. These programs are available on request.

Citations

• A new method for sequencing DNA.
• The use of thin acrylamide gels for DNA sequencing
• A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase
• DNA sequencing with chain-terminating inhibitors

Cited by

• The sequence of sequencers: The history of sequencing DNA
• Evaluation and optimisation of preparative semi‐automated electrophoresis systems for Illumina library preparation
• DNA sequencing [wikipedia]