1974/01/01 by Ernest Jay, Robert A. Bambara, R. Padmanabhan +1 · 1 citation
Biochemistry, Genetics and Molecular Biology · #DNA and Nucleic Acid Chemistry #Advanced biosensing and bioanalysis techniques #RNA and protein synthesis mechanisms #Oligonucleotide #Biology #Nucleotide #Sequence (biology) #DNA #Nucleic acid sequence #Sequence analysis #Bacteriophage #DNA sequencing #Molecular biology #Base pair #Genetics #Biochemistry #Computational biology #Gene
paper · pdf · doi:10.1093/nar/1.3.331
openalex publication_date 1974/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/26
Several electrophoretic and chromatographic systems have been investigated and compared for sequence analysis of oligodeoxyribonucleotides. Three systems were found to be useful for the separation of a series of sequential degradation products resulting from a labeled oligonucleotide: (I) 2-D electrophoresisdagger; (II) 2-D PEI-cellulose; and (III) 2-D homochromatography. System (III) proved generally most informative regardless of base composition and sequence. Furthermore, only in this system will the omission of an oligonucleotide in a series of oligonucleotides be self-evident from the two-dimensional map. The sequence of up to fifteen nucleotides can be determined solely by the characteristic mobility shifts of its sequential degradation products distributed on the two-dimensional map. With this method, ten nucleotides from the double-stranded region adjacent to the left-hand 3'-terminus and seven from the right-hand 3'-terminus of bacteriophage lambda DNA have been sequenced. Similarly, nine nucleotides from the double-stranded region adjacent to the left-hand 3'-terminus and five nucleotides from the right-hand terminus of bacteriophage phi80 DNA have also been sequenced. The advantages and disadvantages of each separation system with respect to sequence analysis are discussed.