The Crystal Structure of Uncomplexed Actin in the ADP State

2001/07/27 by Ludovic R. Otterbein, Philip Graceffa, Roberto Domínguez · 14 citations
Biochemistry, Genetics and Molecular Biology · Medicine · Neuroscience · Chemistry · #Cellular Mechanics and Interactions #Cardiomyopathy and Myosin Studies #Genetic Neurodegenerative Diseases #Actin #Chemistry #Gelsolin #Biophysics #ATP hydrolysis #Adenosine triphosphate #Deoxyribonuclease I #Adenosine diphosphate #Myosin #Actin remodeling #Conformational change #Monomer #Biochemistry #Cytoskeleton #Polymer #Actin cytoskeleton #Platelet aggregation #Biology #ATPase #DNA #Cell #Enzyme #Platelet

paper · doi:10.1126/science.1059700

openalex publication_date 2001/07/27 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01

Abstract

The dynamics and polarity of actin filaments are controlled by a conformational change coupled to the hydrolysis of adenosine 5'-triphosphate (ATP) by a mechanism that remains to be elucidated. Actin modified to block polymerization was crystallized in the adenosine 5'-diphosphate (ADP) state, and the structure was solved to 1.54 angstrom resolution. Compared with previous ATP-actin structures from complexes with deoxyribonuclease I, profilin, and gelsolin, monomeric ADP-actin is characterized by a marked conformational change in subdomain 2. The successful crystallization of monomeric actin opens the way to future structure determinations of actin complexes with actin-binding proteins such as myosin.

Citations

• Atomic structure of the actin: DNase I complex
• 0621 接触界面のガス置換による固体間熱伝達機構の評価とクラッド材への応用(S45-4 原子炉システムおよびその要素技術(4),S45 原子炉システムおよびその要素技術)

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• Actin Structure and Function: What We Still Do Not Understand
• Muscle disease caused by mutations in the skeletal muscle alpha-actin gene (ACTA1)
• Chapter 6 Cell and Molecular Biology of Nuclear Actin
• Water Molecules in the Nucleotide Binding Cleft of Actin: Effects on Subunit Conformation and Implications for ATP Hydrolysis
• Actin polymerization kinetics, cap structure, and fluctuations
• Structure and Filament Dynamics of the pSK41 Actin-like ParM Protein
• Insights into the Cooperative Nature of ATP Hydrolysis in Actin Filaments
• Actin-binding proteins – a unifying hypothesis
• Crystal Structures of Expressed Non-polymerizable Monomeric Actin in the ADP and ATP States
• Actin [wikipedia]