2021/03/18 by Tian Wang, Chunjiao Zhang, Hongyan Zhang +1 · 1 citation
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · #CRISPR and Genetic Engineering #Insect symbiosis and bacterial influences #Plant Virus Research Studies #CRISPR #Genome editing #Biology #Biotechnology #Cas9 #Zinc finger nuclease #Gene #Computational biology #Genetics
paper · doi:10.1021/acs.jafc.1c00104
openalex publication_date 2021/03/18 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/30
Horticultural food crops are important sources of nutrients for humans. With the increase of the global population, enhanced horticulture food crop production has become a new challenge, and enriching their nutritional content has also been required. Gene editing systems, such as zinc finger nucleases, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9), have accelerated crop improvement through the modification of targeted genomes precisely. Here, we review the development of various gene editors and compare their advantages and shortcomings, especially the newly emerging CRISPR/Cas systems, such as base editing and prime editing. We also summarize their practical applications in crop trait improvement, including yield, nutritional quality, and other consumer traits.