The CRISPR/Cas9 system for plant genome editing and beyond

2014/12/20 by Luisa Bortesi, Rainer Fischer · 5 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #CRISPR and Genetic Engineering #Insect symbiosis and bacterial influences #Plant Virus Research Studies

paper · pdf · doi:10.1016/j.biotechadv.2014.12.006

openalex publication_date 2014/12/20 · openalex created_date 2016/06/24 · openalex updated_date 2026/08/01

Abstract

Targeted genome editing using artificial nucleases has the potential to accelerate basic research as well as plant breeding by providing the means to modify genomes rapidly in a precise and predictable manner. Here we describe the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system, a recently developed tool for the introduction of site-specific double-stranded DNA breaks. We highlight the strengths and weaknesses of this technology compared with two well-established genome editing platforms: zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs). We summarize recent results obtained in plants using CRISPR/Cas9 technology, discuss possible applications in plant breeding and consider potential future developments.

Citations

• Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements
• A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

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