2022/04/28 by Yang Liu, Chunling Zhang, Xiao‐Fei Wang +2 · 1 citation
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · #CRISPR and Genetic Engineering #Plant Virus Research Studies #Insect symbiosis and bacterial influences #CRISPR #Genome editing #Transcription activator-like effector nuclease #Cas9 #Zinc finger nuclease #Biotechnology #Biology #Computational biology #Genetics #Gene
paper · doi:10.1016/j.hpj.2022.04.007
openalex publication_date 2022/04/28 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/02
Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated 9 (CRISPR/Cas9) system has recently become one popular technology due to its efficiency, precision, and simplicity compared with other genome editing tools such as Zinc Finger Nucleases (ZFNs) and Transcription Activator Like Effector Nucleases (TALENs). Horticultural crops provide energy and health-keeping nutrients to humankind. Genome-editing technology has become widely adopted in horticultural breeding with the increasing demand for high yield and better-quality horticultural crops. Here, we describe the CRISPR/Cas9 system construction, its optimization, including sgRNA promoter, sgRNA design, Cas9 protein promoter , SpCas9 variants and orthologs, and vector delivery methods. We also summarized the application of this technology in horticultural plants for stress responses enhancement, fruit quality improvement, and cultivation traits modification. This detailed review was compiled to help establish comprehensive understanding of the CRISPR/Cas9 systems and provide a reference for further developing this technology to manipulate horticultural plant traits effectively.