2015/06/24 by Hanfu Xu, David A. O’Brochta · 1 citation
Biochemistry, Genetics and Molecular Biology · Materials Science · #Biology #Biotechnology #Bombyx #Bombyx mori #CRISPR and Genetic Engineering #Computational biology #Gene #Genetics #Genome #Genome editing #Reproductive technology #Silk-based biomaterials and applications #Transcription activator-like effector nuclease #Transgenesis #Transposable element #Viral Infectious Diseases and Gene Expression in Insects
paper · pdf · doi:10.1098/rspb.2015.0487
openalex publication_date 2015/06/24 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/31
Genetic technologies based on transposon-mediated transgenesis along with several recently developed genome-editing technologies have become the preferred methods of choice for genetically manipulating many organisms. The silkworm, Bombyx mori, is a Lepidopteran insect of great economic importance because of its use in silk production and because it is a valuable model insect that has greatly enhanced our understanding of the biology of insects, including many agricultural pests. In the past 10 years, great advances have been achieved in the development of genetic technologies in B. mori, including transposon-based technologies that rely on piggyBac-mediated transgenesis and genome-editing technologies that rely on protein- or RNA-guided modification of chromosomes. The successful development and application of these technologies has not only facilitated a better understanding of B. mori and its use as a silk production system, but also provided valuable experiences that have contributed to the development of similar technologies in non-model insects. This review summarizes the technologies currently available for use in B. mori, their application to the study of gene function and their use in genetically modifying B. mori for biotechnology applications. The challenges, solutions and future prospects associated with the development and application of genetic technologies in B. mori are also discussed.