Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

2010/07/27 by Michelle Christian, Tomáš Čermák, Erin Doyle +5 · 9 citations
Biochemistry, Genetics and Molecular Biology · #CRISPR and Genetic Engineering #RNA Interference and Gene Delivery #Advanced biosensing and bioanalysis techniques

paper · pdf · doi:10.1534/genetics.110.120717

openalex publication_date 2010/07/27 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/22

Abstract

Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.

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