2023/10/10 by Kristaps Kairišs, Natalia Sokolova, Lucie Žílová +7 · 1 voice
Biochemistry, Genetics and Molecular Biology · #Cell Image Analysis Techniques #Advanced Fluorescence Microscopy Techniques #Single-cell and spatial transcriptomics
paper · pdf · doi:10.1101/2023.10.09.561491
openalex publication_date 2023/10/10 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/14
Abstract The development of an organism is orchestrated by the spatial and temporal expression of genes. Accurate visualisation of gene expression patterns in the context of the surrounding tissues offers a glimpse into the mechanisms that drive morphogenesis. We developed correlative light-sheet fluorescence microscopy and X-ray computed tomography approach to map gene expression patterns to the whole organism’s 3D anatomy at cellular resolution. We show that this multimodal approach is applicable to gene expression visualised by protein-specific antibodies and fluorescence RNA in situ hybridisation, offering a detailed understanding of individual phenotypic variations in model organisms. Furthermore, the approach provides a unique possibility to identify tissues together with their 3D cellular and molecular composition in anatomically less-defined in vitro models, such as organoids. We anticipate that the visual and quantitative insights into the 3D distribution of gene expression within tissue architecture, by the multimodal approach developed here, will be equally valuable for reference atlases of model organisms development, as well as for comprehensive screens and morphogenesis studies of in vitro models.