Light-sheets and smart microscopy, an exciting future is dawning

2023/05/09 by Stephan Daetwyler, Reto Fiolka · 1 voice · 3 citations
Biochemistry, Genetics and Molecular Biology · #Advanced Fluorescence Microscopy Techniques #Cell Image Analysis Techniques #Single-cell and spatial transcriptomics

paper · pdf · doi:10.1038/s42003-023-04857-4

openalex publication_date 2023/05/09 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01

Abstract

Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods. In this review, we discuss current and future developments in light-sheet fluorescence microscopy that we expect to further expand its capabilities. This includes smart and adaptive imaging schemes to overcome traditional imaging trade-offs, i.e., spatiotemporal resolution, field of view and sample health. In smart microscopy, a microscope will autonomously decide where, when, what and how to image. We further assess how image restoration techniques provide avenues to overcome these tradeoffs and how "open top" light-sheet microscopes may enable multi-modal imaging with high throughput. As such, we predict that light-sheet microscopy will fulfill an important role in biomedical and clinical imaging in the future.

Citations

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Cited by

• Visualisation of gene expression within the context of tissues: an X-ray computed tomography-based multimodal approach
• There is more to scanning than meets the eye: Raster Image Correlation Spectroscopy
• Distinct actin microfilament localization during early cell plate formation through deep learning-based image restoration

Discussions

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