Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays

2023/09/14 by Andrew Santiago-Frangos, Andrew Santiago‐Frangos, William S. Henriques +10 · 1 voice
Biochemistry, Genetics and Molecular Biology · Environmental Science · #Bacteriophages and microbial interactions #CRISPR and Genetic Engineering #RNA and protein synthesis mechanisms

paper · doi:10.1038/s41594-023-01097-2

crossref issued 2023/09/14 · crossref published 2023/09/14 · crossref published-online 2023/09/14 · openalex publication_date 2023/09/14 · crossref created 2023/09/14 · crossref published-print 2023/11/01 · crossref deposited 2023/11/13 · openalex created_date 2025/10/10 · crossref indexed 2026/04/07 · openalex updated_date 2026/08/01

Citations

• The origin and behavior of mutable loci in maize
• MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability
• UCSF ChimeraX: Structure visualization for researchers, educators, and developers
• Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction
• ColabFold: making protein folding accessible to all

Discussions

• Cas1 and Cas2 are the hallmark proteins of CRISPR immunity. However, in the type I-F CRISPR system, Cas2 is fused to Cas3, a helicase/nuclease. Previously, @asantiagofrangos.bsky.social showed Cas1- [bsky, 0 points, 1 comments]