2025/06/06 by Chan Zhao, Yu‐Hua Xin, Qing Liu · 1 voice
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · Environmental Science · #Genomics and Phylogenetic Studies #Microbial Community Ecology and Physiology #Probiotics and Fermented Foods
paper · doi:10.1099/ijsem.0.006799
openalex publication_date 2025/06/06 · openalex created_date 2025/06/07 · openalex updated_date 2026/07/22
Three Gram-stain-negative, rod-shaped, motile bacterial strains, each with a single polar flagellum, designated LB3P38 T , LT1P18 T and ZB1P45 T , were isolated from glacier samples on the Tibetan Plateau, P.R. China. These strains grew at temperatures ranging from −2 to 32 °C (optimum 25–30 °C) and at pH values of 5.0–11.0 (optimum pH 7.0). The similarities of 16S rRNA gene sequences among these strains ranged from 99.67% to 99.93%. Phylogenetic analysis based on 16S rRNA gene sequences confirmed their affiliation with the genus Pseudomonas . Phylogenomic analysis positioned these strains in close relation to Pseudomonas svalbardensis PMCC 200367 T and Pseudomonas frederiksbergensis LMG 19851 T . Average nucleotide identity values and digital DNA–DNA hybridization values between these strains and other type strains of the genus Pseudomonas were below 94.6% and 63.4%, respectively. The predominant fatty acids identified in these strains were C 16:0 , C 17:0 cyclo and summed feature 3 (C 16:1 ω7 c and/or C 16:1 ω6 c). Based on the combined phenotypic and phylogenetic evidence, each strain is proposed to represent a novel species within the genus Pseudomonas , with the names Pseudomonas lyxosi sp. nov. (type strain=LB3 P38 T =CGMCC 1.11284 T =JCM 37126 T ), Pseudomonas arabinosi sp. nov. (type strain=LT1 P18 T =CGMCC 1.11310 T =JCM 37131 T ) and Pseudomonas frigoris sp. nov. (type strain=ZB1 P45 T =CGMCC 1.23235 T =JCM 37141 T ) proposed.