Nucleoside Phosphorylases make N7-xanthosine

2024/04/29 by Sarah Westarp, Felix Brandt, Lena Neumair +7 · 1 voice
Biochemistry, Genetics and Molecular Biology · Medicine · #Biochemical and Molecular Research #HIV/AIDS drug development and treatment #Peptidase Inhibition and Analysis

paper · pdf · doi:10.1038/s41467-024-47287-4

openalex publication_date 2024/04/29 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

Modern, highly evolved nucleoside-processing enzymes are known to exhibit perfect regioselectivity over the glycosylation of purine nucleobases at N9. We herein report an exception to this paradigm. Wild-type nucleoside phosphorylases also furnish N7-xanthosine, a "non-native" ribosylation regioisomer of xanthosine. This unusual nucleoside possesses several atypical physicochemical properties such as redshifted absorption spectra, a high equilibrium constant of phosphorolysis and low acidity. Ultimately, the biosynthesis of this previously unknown natural product illustrates how even highly evolved, essential enzymes from primary metabolism are imperfect catalysts.

Citations

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Discussions

• Either way, I'm all for giving credit to reviewers for useful suggestions that add to the quality for a manuscript. We do so pretty consistently wherever it applies. For instance... (From doi.org/10. [bsky, 3 points, 1 comments]

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