2000/01/01 by DAVID R. SELL, NANETTE R. KLEINMAN, VINCENT M. MONNIER
paper · doi:10.1096/fasebj.14.1.145
ABSTRACT In 1988, the National Institute on Aging launched a 10‐year program aimed at identification of biomarkers of aging. Previous results from our laboratory showed that pentosidine, an advanced glycation product, formed in skin collagen at a rate inversely related to maximum life span across several mammalian species. As part of the Biomarkers Program, we investigated the hypothesis that longitudinal determination of glycation and glycoxidation rates in skin collagen could predict longevities in ad libitum ‐fed (AL) and caloric restricted (CR) mice. C57BL/6NNia male mice were biopsied at age 20 months and at natural death. Glycation (furosine method) was assessed by gas chromatography/mass spectrometry (GC/MS) and the glycoxidation products carboxymethyllysine (CML) and pentosidine were determined by GC/MS and HPLC, respectively. CR vs. AL significantly ( P P P r =0.54, P =0.002). The finding that markers of skin collagen glycation and glycoxidation rates can predict early deaths in AL and CR C57BL/6NNia mice strongly suggests that an age‐related deterioration in glucose tolerance is a life span‐determining process.—Sell, D. R., Kleinman, N. R., Monnier, V. M. Longitudinal determination of skin collagen glycation and glycoxidation rates predicts early death in C57BL/6NNIA mice. FASEB J. 14, 145–156(2000)