1991/01/01 by Robert H. Braun, Braun, R. H., David W. M. Leung +1
Biochemistry, Genetics and Molecular Biology · Agricultural and Biological Sciences · #Plant tissue culture and regeneration #Flowering Plant Growth and Cultivation #Plant Molecular Biology Research
paper · pdf · doi:10.26021/384
Braun, R.H. & Leung, D.W.M. (1991). Establishment and clonal propagation of in vitro plantlets of Leptospermum scoparium. New Zealand Natural Sciences 18: 39-43. Nodal segments of manuka (Leptospernum scoparium, J.R. et G. Forst.) were surface sterilised and initiated into aseptic culture. Optimal conditions for culture establishment were investigated. Explants grew well on low salt media without the inclusion of growth factors or carbon source and rooting occurred readily in such conditions. The presence of auxins had no significant enhancing effect on root formation or elongation compared to control. Cytokinins promoted bud formation but inhibited their elongation. In vitro plantlets could be readily multiplied. Roots formed were shown by sectioning to be of pericycilc origin.