2012/03/05 by H. Polzerová, Polzerová, Hana, Marie Greplová +3
Biochemistry, Genetics and Molecular Biology · #Plant tissue culture and regeneration
paper · pdf · doi:10.5073/jabfq.2011.084.001
openalex publication_date 2012/03/05 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
The multiplication protocol of the medicinal plant Lepidium meyenii was established through axillary bud proliferation. Multiplication was initiated on modified MS supplemented with a combination of zeatin (2 μM) and gibberellic acid 3 (0.3 μM). The average number of proliferated shoots was 5.3 per segment. Shoots were rooted on modified MS medium with 9.8 μM of indole-3-butyric acid and 3 μM of a-naphthalene acetic acid. In vivo rooting was also successful and 94 % regenerated plantlets were acclimatized.