1998/07/01 by Kee Yoeup Paek, Kee‐Yoeup Paek, Toyoki Kozai
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · #Flowering Plant Growth and Cultivation #Plant Pathogens and Fungal Diseases #Plant tissue culture and regeneration
paper · pdf · doi:10.21273/horttech.8.3.283
openalex publication_date 1998/07/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/06/11
We report the results of serial studies aimed at clarifying several factors affecting organogenesis in rhizome culture of temperate Cymbidium species and their hybrids. The growth patterns and regeneration ability of rhizomes derived from asymbiotic seed or shoot tip culture vary according to media composition, kinds and concentrations of plant growth regulators, culture conditions, and species and varieties. N 6 -benzyladenine was the best cytokinin for inducing shoot formation, for switching rhizome tissues into protocorm-like bodies, and for directly forming multiple shoots from branched rhizomes. Activated charcoal appeared to be necessary for producing healthy plantlets and for stimulating shoot growth at levels of 0.1% to 0.3% but concomitantly decreased rhizome growth. Sucrose at 5% was the most effective concentration for shoot induction from rhizomes. The above results support the conclusion that organogenic pathways between tropical, subtropical, and temperate Cymbidium species may be controlled by the genetic backgrounds of the species or cultivars.