2025/04/30 by Roßbach, Bernd, Rißler, Eleonore, Budnik, Lygia Therese +4
#1-Propyl bromide #1-Propylbromid #1‐Bromopropane #1‐Brompropan #2-Propyl bromide #2-Propylbromid #2‐Bromopropane #2‐Brompropan #543 Analytical chemistry #543 Analytische Chemie #571.95 Toxicology #571.95 Toxikologie #616.9803 Betriebs- und Arbeitsmedizin #616.9803 Industrial and occupational medicine #Allgemeinbevölkerung #Analysen in biologischem Material #Analysenmethode #Analyses in Biological Materials #Arbeiter #Arbeitnehmer #Arbeitsplatz #Arbeitsplatzbelastung #Biomonitoring #Biomonitoringmethode #Dynamische Festphasenextraktion #HBM #HS-SPDE-GC-MS #Headspace-solid phase dynamic extraction-Gaschromatographie mit Massenspektrometrie #Hintergrundbelastung #Humanbiomonitoring #Intoxikation #Methode #Umweltbelastung #Urin #Vergiftung #analytical method #background exposure #biological monitoring #biologisches Monitoring #biomonitoring #biomonitoring method #employee #environmental exposure #general population #headspace-solid phase dynamic extraction gas chromatography with mass spectrometry #human biomonitoring #intoxication #method #poisoning #urine #worker #workplace #workplace exposure
paper · doi:10.34865/bi10694d0022_w
The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area verified the presented biomonitoring method. The analytical method described herein permits the sensitive determination of both unmetabolised 1‐bromopropane and 2‐bromopropane in urine. Prior to the determination using GC‐MS in the Single‐Ion‐Mode (SIM), the analytes are extracted and enriched using Headspace Solid Phase Dynamic Extraction (SPDE). To this end, the urine samples are incubated at 50 °C and the analytes are extracted from the gas phase using SPDE. The headspace extract is then injected into the gas chomatograph for separation and mass spectrometric analysis. Calibration standards are prepared in urine and processed in the same way as the samples to be analysed. Deuterated benzene is used as the internal standard (IS).