Insights into the mechanisms of electromediated gene delivery and\n application to the loading of giant vesicles with negatively charged\n macromolecules

2011/02/03 by Thomas Portet, Portet, Thomas, Cyril Favard +7
Biochemistry, Genetics and Molecular Biology · #RNA Interference and Gene Delivery #Lipid Membrane Structure and Behavior #Advanced biosensing and bioanalysis techniques

paper · pdf · doi:10.48550/arxiv.1102.0610

Abstract

We present experimental results regarding the electrotransfer of plasmid DNA\ninto phosphatidylcholine giant unilamellar vesicles (GUVs). Our observations\nindicate that a direct entry is the predominant mechanism of electrotransfer. A\nquantitative analysis of the DNA concentration increments inside the GUVs is\nalso performed, and we find that our experimental data are very well described\nby a simple theoretical model in which DNA entry is mostly driven by\nelectrophoresis. Our theoretical framework allows for the prediction of the\namount of transfered DNA as a function of the electric field parameters, and\nthus paves the way towards a novel method for encapsulating with high\nefficiency not only DNA, but any negatively charged macromolecule into GUVs.\n

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