2025/01/01 by Hoppe, Hans-Wolfgang, Zarniko, Mareike, Müller, Johannes +3
Biochemistry, Genetics and Molecular Biology · Medicine · #Carcinogens and Genotoxicity Assessment #Occupational exposure and asthma #Alcohol Consumption and Health Effects
paper · doi:10.34865/bi163404e2218_w
The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area verified the present biomonitoring method. The described analytical method allows the specific and sensitive determination of methyl tert‐butyl ether (MTBE) in blood and urine of persons occupationally exposed to this substance. The volatile MTBE is determined using static headspace GC‐MS. Before analysing an aliquot of the headspace, the urine and blood samples are diluted 1:10 with water, spiked with internal standard and incubated for 45 min at 60 °C. The isotope‐labelled d3‐MTBE serves as internal standard. Aqueous standard solutions are used for calibration. The method was extensively validated and the reliability data were confirmed by an independent laboratory, which has established and cross‐checked the whole procedure.