Biomolecular resource utilization in elementary cell-free gene circuits

2013/06/30 by Dan Siegal‐Gaskins, Vincent Noireaux, Siegal-Gaskins, Dan +3
Biochemistry, Genetics and Molecular Biology · #Biomolecules (q-bio.BM) #CRISPR and Genetic Engineering #FOS: Biological sciences #Gene Regulatory Network Analysis #Molecular Networks (q-bio.MN) #RNA and protein synthesis mechanisms

paper · pdf · doi:10.48550/arxiv.1307.0178

openalex publication_date 2013/06/30 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

We present a detailed dynamical model of the behavior of transcription-translation circuits in vitro that makes explicit the roles played by essential molecular resources. A set of simple two-gene test circuits operating in a cell-free biochemical 'breadboard' validate this model and highlight the consequences of limited resource availability. In particular, we are able to confirm the existence of biomolecular 'crosstalk' and isolate its individual sources. The implications of crosstalk for biomolecular circuit design and function are discussed.

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