2021/12/09 by Priya Chakraborty, Chakraborty, Priya, Sayantari Ghosh +1
Biochemistry, Genetics and Molecular Biology · #FOS: Biological sciences #Gene Regulatory Network Analysis #Molecular Networks (q-bio.MN) #Quantitative Methods (q-bio.QM) #RNA and protein synthesis mechanisms #Receptor Mechanisms and Signaling
paper · pdf · doi:10.48550/arxiv.2112.04985
openalex publication_date 2021/12/09 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
Synthetic genetic circuits are implemented in living cells for their\noperation. During gene expression, proteins are produced from the respective\ngenes, by formation of complexes through the process of transcription and\ntranslation. In transcription the circuit uses RNAP, etc. as resource from the\nhost cell and in translation, ribosome, tRNA and other cellular resources are\nsupplied to the operating circuit. As the cell contains these resources in\nlimited number, the circuit can suffer from unprecedented resource competition\nwhich might destroy the circuit functionality, or introduce some emergent\nresponses. In this paper, we have studied a three-gene motif under resource\ncompetition where interesting behaviour, similar to regulatory responses occur\ndue to limited supply of necessary resources. The system of interest exhibits\nprominent changes in behaviour which can be observed experimentally. We focus\non two specific aspects, namely, dynamic range and half-induction point, which\ninherently describe the circuit functionalities, and can be affected by\ncorresponding resource affinity and availability.\n