Programmable CRISPR-responsive smart materials

2019/08/23 by Max A. English, Luis R. Soenksen, Raphaël V. Gayet +6 · 33 citations
Biochemistry, Genetics and Molecular Biology · Engineering · #Advanced biosensing and bioanalysis techniques #Advanced Memory and Neural Computing #CRISPR and Genetic Engineering

paper · doi:10.1126/science.aaw5122

Abstract

Stimuli-responsive materials activated by biological signals play an increasingly important role in biotechnology applications. We exploit the programmability of CRISPR-associated nucleases to actuate hydrogels containing DNA as a structural element or as an anchor for pendant groups. After activation by guide RNA-defined inputs, Cas12a cleaves DNA in the gels, thereby converting biological information into changes in material properties. We report four applications: (i) branched poly(ethylene glycol) hydrogels releasing DNA-anchored compounds, (ii) degradable polyacrylamide-DNA hydrogels encapsulating nanoparticles and live cells, (iii) conductive carbon-black-DNA hydrogels acting as degradable electrical fuses, and (iv) a polyacrylamide-DNA hydrogel operating as a fluidic valve with an electrical readout for remote signaling. These materials allow for a range of in vitro applications in tissue engineering, bioelectronics, and diagnostics.

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• Smart/stimuli-responsive hydrogels: Cutting-edge platforms for tissue engineering and other biomedical applications. [europepmc]
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• Discovery of Diverse CRISPR-Cas Systems and Expansion of the Genome Engineering Toolbox. [europepmc]
• Chemical and Biological Engineering Strategies to Make and Modify Next-Generation Hydrogel Biomaterials. [europepmc]
• Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis and transforms DNA/RNA sensing. [europepmc]

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