2022/03/31 by Göen, Thomas, Schäferhenrich, Anja, Taghikhani, Emir +3
#1 #2 #2-tetrafluorethoxy)phenyl]carbamoyl]-2 #2-trifluor-2-(trifluormethoxy)ethoxy]phenyl]carbamoyl]-2 #3 #3-hexafluorpropoxy)phenyl]carbamoyl]-2 #4-difluorphenyl)carbamoyl]-2 #5-Dichlor-2 #5-Dichlor-2-fluor-4-(1 #5-Dichlor-4-(1 #5-Dichlor-4-[3-chlor-5-(trifluormethyl)pyridin-2-yl]oxyphenyl]carbamoyl]-2 #5-bis(trifluormethyl)phenyl]carbamoyl]-2 #6-Difluor-N-[[4-(trifluormethyl)phenyl]carbamoyl]benzamid #6-Difluorbenzoesäure #6-difluorbenzamid #6-difluorobenzoic acid #Allgemeinbevölkerung #Analysen in biologischem Material #Analysenmethode #Analyses in Biological Materials #Arbeiter #Arbeitnehmer #Arbeitsplatz #Arbeitsplatzbelastung #Benzoylharnstoffverbindungen #Biomonitoring #Biomonitoringmethode #Biozide #Bistrifluron #Chlorfluazuron #Diflubenzuron #Fluazuron #Flucycloxuron #Flufenoxuron #GC-MS #Gas chromatography-mass spectrometry #Gaschromatographie #Gaschromatographie-Massenspektrometrie #HBM #Hexaflumuron #Hintergrundbelastung #Humanbiomonitoring #Intoxikation #Lufenuron #Massenspektrometrie #Methode #N-[(3 #N-[(4-Chlorphenyl)carbamoyl]-2 #N-[[2 #N-[[2-Chlor-3 #N-[[3 #N-[[3-Chlor-4-[1 #N-[[4-Chlor-3-[3-chlor-5-(trifluormethyl)pyridin-2-yl]oxyphenyl]carbamoyl]-2 #N-[[4-[2-Chlor-4-(trifluormethyl)phenoxy]-2-fluorphenyl]carbamoyl]-2 #N-[[4-[[(E)-[(4-Chlorphenyl)cyclopropylmethyliden]amino]oxymethyl]phenyl]carbamoyl]-2 #Novaluron #Noviflumuron #Penfluron #Pestizide #Teflubenzuron #Umweltbelastung #Urin #Vergiftung #analytical method #background exposure #benzoylurea compounds #biocides #biological monitoring #biologisches Monitoring #biomonitoring #biomonitoring method #employee #environmental exposure #gas chromatography #general population #human biomonitoring #intoxication #mass spectrometry #method #pesticides #poisoning #urine #worker #workplace #workplace exposure
paper · doi:10.34865/bi3850e7_1or
The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and verified the presented biomonitoring method.The analytical method described herein allows for the sensitive and specific determination of 2,6‑difluorobenzoic acid (2,6‑DFBA) in the urine of individuals occupationally exposed to 2,6‑difluorosubstituted benzoylurea compounds. 2,6‑DFBA is determined by gas chromatography with mass-spectrometric detection (GC‑MS) after derivatisation. To this end, urine samples are mixed with an internal standard (ISTD), acidified, and purified using solid-phase extraction (SPE). The samples are derivatised with N‑methyl-N‑tert‑butyldimethylsilyltrifluoroacetamide (MTBSTFA) and the analyte concentration is quantified by GC‑MS. The ISTD used is 5‑bromo-2‑fluorobenzoic acid. Calibration is performed using standard solutions prepared in pooled urine.