Photogenerated singlet oxygen damages cells in optical traps

2009/11/24 by S.D. Zakharov N.C. Thanh, Thanh, S. D. Zakharov N. C.
Biochemistry, Genetics and Molecular Biology · Physics and Astronomy · #Advanced Fluorescence Microscopy Techniques #Biological Physics (physics.bio-ph) #FOS: Physical sciences #Laser-Matter Interactions and Applications #Optics (physics.optics) #Orbital Angular Momentum in Optics

paper · pdf · doi:10.48550/arxiv.0911.4651

openalex publication_date 2009/11/24 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28

Abstract

The origin of photodamage of single cells confined in laser tweezers is determined by comparison of action spectra with these recorded for a photoinduced response of cell populations. Triplet-singlet transitions in dissolved oxygen provide a common cause of both phenomena. Such transitions are forbidden in isolated O2 molecules, but occur in solutions due to the formation of temporary oxygen-solvent complexes. In dependence on the photon energy oxygen molecules may be excited alone or in combination with roto-vibrational excitations in H2O. The photodamage is a consequence of a toxic action produced by the singlet oxygen 1O2 (1.DELTA.gO2.).

Citations

• Acceleration and Trapping of Particles by Radiation Pressure

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