A Beauveria phylogeny inferred from nuclear ITS and EF1- sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs
2005/01/01 by Stephen A. Rehner, S. A. Rehner, Ellen Buckley +1 · 1,481 citations
Agricultural and Biological Sciences · Veterinary · #Ascomycota #Bassiana #Beauveria #Beauveria bassiana #Biological pest control #Biology #Botany #Clade #Conidium #Cordyceps #Entomopathogenic Microorganisms in Pest Control #Gene #Genetics #Helminth infection and control #Hypocreales #Monophyly #Nematode management and characterization studies #Phylogenetic tree #Phylogenetics #Polyphyly #Species complex #Zoology
paper · doi:10.3852/mycologia.97.1.84
published in Mycologia 97(1), 84-98 (Taylor & Francis)
openalex publication_date 2005/01/01 · openalex created_date 2016/06/24 · openalex updated_date 2026/07/31
Abstract
Beauveria is a globally distributed genus of soil-borne entomopathogenic hyphomycetes of interest as a model system for the study of entomopathogenesis and the biological control of pest insects. Species recognition in Beauveria is difficult due to a lack of taxonomically informative morphology. This has impeded assessment of species diversity in this genus and investigation of their natural history. A gene-genealogical approach was used to investigate molecular phylogenetic diversity of Beauveria and several presumptively related Cordyceps species. Analyses were based on nuclear ribosomal internal transcribed spacer (ITS) and elongation factor 1-alpha (EF1-alpha) sequences for 86 exemplar isolates from diverse geographic origins, habitats and insect hosts. Phylogenetic trees were inferred using maximum parsimony and Bayesian likelihood methods. Six well supported clades within Beauveria, provisionally designated A-F, were resolved in the EF1-alpha and combined gene phylogenies. Beauveria bassiana, a ubiquitous species that is characterized morphologically by globose to subglobose conidia, was determined to be non-monophyletic and consists of two unrelated lineages, clades A and C. Clade A is globally distributed and includes the Asian teleomorph Cordyceps staphylinidaecola and its probable synonym C. bassiana. All isolates contained in Clade C are anamorphic and originate from Europe and North America. Clade B includes isolates of B. brongniartii, a Eurasian species complex characterized by ellipsoidal conidia. Clade D includes B. caledonica and B. vermiconia, which produce cylindrical and comma-shaped conidia, respectively. Clade E, from Asia, includes Beauveria anamorphs and a Cordyceps teleomorph that both produce ellipsoidal conidia. Clade F, the basal branch in the Beauveria phylogeny includes the South American species B. amorpha, which produces cylindrical conidia. Lineage diversity detected within clades A, B and C suggests that prevailing morphological species concepts underestimate species diversity within these groups. Continental endemism of lineages in B. bassiana s.l. (clades A and C) indicates that isolation by distance has been an important factor in the evolutionary diversification of these clades. Permutation tests indicate that host association is essentially random in both B. bassiana s.l. clades A and C, supporting past assumptions that this species is not host specific. In contrast, isolates in clades B and D occurred primarily on coleopteran hosts, although sampling in these clades was insufficient to assess host affliation at lower taxonomic ranks. The phylogenetic placement of Cordyceps staphylinidaecola/bassiana, and C. scarabaeicola within Beauveria corroborates prior reports of these anamorph-teleomorph connections. These results establish a phylogenetic framework for further taxonomic, phylogenetic and comparative biological investigations of Beauveria and their corresponding Cordyceps teleomorphs.
Citations
Cited by
- Amylocorticiales ord. nov. and Jaapiales ord. nov.: Early diverging clades of Agaricomycetidae dominated by corticioid forms
- Atractosporocybe, Leucocybe and Rhizocybe : three new clitocyboid genera in the Tricholomatoid clade (Agaricales) with notes on Clitocybe and Lepista
- Three new Pochonia taxa (Clavicipitaceae) from soils in Japan
- A new species of Samsoniella ( Cordycipitaceae : Hypocreales ) on the herald moth, Scoliopteryx libatrix ( Erebidae : Noctuoidea ), from caves in the British Isles
- Taxonomy and phylogeny of Postia.Multi-gene phylogeny and taxonomy of the brown-rot fungi: Postia (Polyporales, Basidiomycota) and related genera
- Fenestelloid clades of the Cucurbitariaceae
- Malassezia vespertilionis sp. nov.: a new cold-tolerant species of yeast isolated from bats
- One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes
- Culturable mycobiota from Karst caves in China, with descriptions of 20 new species
- Multi-gene phylogeny and taxonomy of Amauroderma s. lat. (Ganodermataceae)
- A re-evaluation of Neotropical Junghuhnia s.lat. (Polyporales, Basidiomycota) based on morphological and multigene analyses
- A fifty-locus phylogenetic analysis provides deep insights into the phylogeny of Tricholoma (Tricholomataceae, Agaricales)
- Global diversity of the Ganoderma lucidum complex (Ganodermataceae, Polyporales) inferred from morphology and multilocus phylogeny
- Genomic Comparisons of Two Armillaria Species with Different Ecological Behaviors and Their Associated Soil Microbial Communities
- Pseudobaeosporoideae, a new subfamily within the Tricholomataceae for the genus Pseudobaeospora (Agaricales, Tricholomatineae) based on morphological and molecular inference
- Inocybaceae of Pakistan: a checklist and description of two novel Inocybe species using morphological and multi-locus phylogenetic evidence
- Morphological and phylogenetic analyses revealed Fissuracium ellipsoideum gen. et sp. nov. (Amylocorticiaceae, Amylocorticiales) from southwest China
- Multi-locus molecular phylogenetic analysis reveals four new species and a new record of Ophiocordyceps (Ophiocordycipitaceae, Hypocreales) on dipteran hosts in Thailand
- The multi-locus phylogeny reveals a cryptic species within the <i>Suillus grevillei</i> complex in eastern Eurasia
- Unexpected richness and distinct patterns of Morchella (Ascomycota) species diversity in Chongqing, a notable “Furnace City”: unveiling rich diversity in hot regions
- Non-lichenized Cytosporella, including C. fuligomixta sp. nov., and related plant-associated and fungicolous genera are close to foliicolous, lichenized fungi (Ascomycota, Graphidales)
- Three new species and a new synonym of Conocybe section Ochromarasmius subsection Dumetorae (Bolbitiaceae, Agaricales) from northern China
- Examination of mycoparasites reveals a new type of host-parasite interface and rearranges the taxonomy of Occultifur and Microsporomyces ( Cystobasidiomycetes , Basidiomycota )
- <i>Russula nuda</i>, a new species of <i>Russula</i> subsect. <i>Xerampelinae</i> (<i>Russulaceae</i>) from Japan
- Species diversity and systematic taxonomy of Sarcosomataceae (Ascomycota, Pezizales), with an emphasis on subtropical regions of China
- Three new species of Agaricus (Agaricaceae, Agaricales) from southern China
- Two new species of Micropsalliota (Agaricales, Agaricaceae) from subtropical regions of China
- Two new saprobic species of Helicosporium and Dictyosporium and a new host record of Neohelicomyces guizhouensis isolated from Phellodendron amurense (Rutaceae, Tracheophyta) in China
- A subgeneric revision of the genus Suillus (Suillaceae, Boletales) and novel taxa from Eastern Asia based on morphology and multigene phylogenies
- Two new species and one new record of Perennicordyceps (Polycephalomycetaceae, Hypocreales) from China
- Phylogenetic and phenotypic evidence of Bannoa (Erythrobasidiales, Erythrobasidiaceae) revealing one new genus and four new species in China
- Taxonomic and phylogenetic insights into dipteran-parasitizing Ophiocordyceps: Descriptions of two new species and a new record from China and Laos
- Two novel Tuber species (Tuberaceae, Pezizales) from southwestern China based on morphological and molecular evidence
- Entomopathogenic fungal endophytes
- Morpho-phylogenetic analyses of two novel edible mushrooms from China and a mini review of Lyophyllum (Agaricales, Lyophyllaceae) cultivation and bioactivities
- Two new species of Hyaloambispora and Paramirandina (Microthyriaceae, Microthyriales) from freshwater habitats in China
- Integrating morphology, phylogeny, substrate, and distribution: clarifying the major phylogenetic framework of Pluteus sect. Hispidoderma (Agaricales, Pluteaceae) and describing 18 species
- Pseudobaorangia (Boletaceae, Boletales), a new genus for Boletus lakhanpalii
- Multigene phylogenetic analyses and morpho-anatomical characterization revealed two new species of Xylariomycetidae from Yunnan, China
- Additions to Didymosphaeriaceae: two novel species of Chromolaenicola crataegicola and Paraphaeosphaeria fulva with notes on antibacterial and antifungal activities
- Novel Helicotubeufia and Tubeufia (Tubeufiaceae, Tubeufiales) species from terrestrial habitats in Hainan Province, China
- The Ganoderma curtisii lineage (Basidiomycota, Polyporaceae) in the Neotropics: Ganoderma mexicurtisii sp. nov. from pine-oak forests in Mexico
- Two new species of Gymnopus sect. Levipedes (Omphalotaceae, Agaricales) from Central and North China
- <i>Neostagonosporella sasae</i> (<i>Phaeosphaeriaceae</i>, <i>Pleosporales</i>), a newly described species from bamboo leaves in Japan
- Molecular phylogeny and morphology reveal four novel species in Cordycipitaceae in China
- A new psychrophilic yeast of Kriegeriaceae (Kriegeriales) isolated from lichen in the Arctic, with the description of Lichenia svalbardensis gen. et sp. nov.
- Phylogeny and taxonomy of Nigroporus (Polyporales, Basidiomycota) with four new species from Asia and Oceania
- Additions to the saprobic fungi (Ascomycota) associated with macadamia trees from the Greater Mekong Subregion
- Taxonomic novelties in Helminthosphaeriaceae: morphological and phylogenetic evidence for Obovatispora gen. nov. and a new species from Polygonatum in Guizhou Province, China
- Taxonomy and molecular evaluation of the Halosphaeriaceae (Microascales, Hypocreomycetidae)
- Novel Bionectriaceae (Hypocreales) taxa associated with ferns in southwestern China: morpho-phylogenetic description of two new species and one new host record
- Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community
- Insect pathogens as biological control agents: Back to the future
- Multi-gene phylogeny and morphological evidence reveal two novel species and two new records of Acrogenospora (Minutisphaerales, Acrogenosporaceae) from aquatic habitats in mainland China
- New species and records of Cytospora (Cytosporaceae, Diaporthales) from tree branches in Hebei Province, China
- Exploring the dominant endophytic pleosporalean fungi in Poaceae plants: taxonomic novelties within the suborder Massarineae
- Two new species and one asexual morph record of Paraisaria (Ophiocordycipitaceae, Hypocreales) from China
- Morphological and phylogenetic analyses reveal two new species of Neohelicomyces (Tubeufiales, Tubeufiaceae) from China
- A phylogenetic and morphological study of the genus Dermoloma (Agaricales, Tricholomataceae) in Europe and North America exposes inefficiency of opportunistic species descriptions
- Emendation of morphology and infrageneric standards of Parasola (Psathyrellaceae, Agaricales) and its species diversity in China
- New species and records of Phaeobotryon (Botryosphaeriales, Botryosphaeriaceae) from Larix in China
- Two new species of Neohelicomyces (Tubeufiaceae, Tubeufiales) from southern China revealed by morphological and multi-locus phylogenetic analyses
- Morphological and phylogenetic analyses reveal three new species of Chaetosphaeriaceae from China
- Two novel species of Neohelicomyces (Tubeufiaceae, Tubeufiales) from Guizhou Province, China
- Three new species of Gnomoniopsis and Ternstroemiomyces (Diaporthales, Sordariomycetes) from China
- Contributions of rpb2 and tef1 to the phylogeny of mushrooms and allies (Basidiomycota, Fungi)
- What are the 100 most cited fungal genera?
- Discovery of a novel Keithomyces species isolated from the sclerotia and a new Papiliomyces from Southwest China: Integrated taxonomy based on morphology and phylogeny
- New additions to Sympoventuriaceae (Venturiales, Dothideomycetes): three new species from China
- Morphological and phylogenetic analyses revealed Cystidichaete alba gen. et sp. nov. (Phanerochaetaceae, Polyporales) from Southwest China
- Morphological and phylogenetic analyses reveal two new species of Niesslia (Niessliaceae, Hypocreales) from China
- Studies in phylogeny and divergence times of Irpicaceae and Meripilaceae (Polyporales, Basidiomycota), with an emphasis on Ceriporia and Meripilus including ten new species
- Additions to woody litter fungi of Byssosphaeria, Phaeoseptum and Pseudothyridariella (Pleosporales, Ascomycota) from China
- Morphological and phylogenetic analyses reveal two new species of Tubeufia (Tubeufiales, Tubeufiaceae) from freshwater habitats in China
- Morphology, multilocus phylogeny, and toxin analysis reveal two new species of Amanita section Amanita (Amanitaceae) from China
- Morpho-phylogenetic evidence reveals novel Bambusicolous fungi from Guizhou Province, China
- New fungal genus, three novel species and one new record from mangroves, with reclassification of Melanconiella (Melanconiellaceae) species
- Lijiangomyces laojunensis gen. et sp. nov. (Mytilinidiaceae), and Sclerococcum stictae (Dactylosporaceae), a new lichenicolous species from Yunnan, China
- Three new species and a new record of Conocybe section Pilosellae (Bolbitiaceae, Agaricales) from Jilin Province, China
- A new genus and species of <i>Phialocephala</i>-like fungi with cordiform conidia in <i>Chaetosphaeriaceae</i>
- A Survey of Canker-Associated Pathogens in Declining Cold-Hardy Interspecific Hybrid Grapevines in Iowa
- Taxonomy and phylogeny of Pseudochaetosphaeronema associated with rubber trees from Yunnan Province, China
- Taxonomic revision of Conobolbitina (Bolbitiaceae, Agaricales) based on collections from Jilin Province, China
- Taxonomic novelties in Dictyosporiaceae and Pleurotremataceae (Dothideomycetes, Ascomycota): Two new species and three new host reports in the coastal region of Guangdong Province, China
- Morphological and phylogenetic analyses reveal novel entomopathogenic fungi infecting scale insects and aphids in China
- Five novel species of Curvularia (Pleosporales, Pleosporaceae) isolated from turfgrasses
- Two new species of Chionosphaera and Kurtzmanomyces (Chionosphaeraceae, Agaricostilbomycetes) isolated from China
- Two novel Helicosporium species (Tubeufiaceae, Tubeufiales) from southern China based on morphological and molecular evidence
- Two new species of Nigropunctata and the first report of sexual morph of Melanographium citri (Pallidoperidiaceae, Xylariales) from south-western China
- The phylogeny and taxonomy of Violella (Tephromelataceae, lichenized Ascomycota), including a new species from China
- Biogeography and species diversity of freshwater Savoryellomycetidae ( Sordariomycetes ) fungi
- Morpho-phylogenetic evidence reveals novel hyphomycetous fungi on medicinal plants in Southwestern China
- Overview of hirsutella-like anamorphs in Ophiocordyceps (Sordariomycetes, Ophiocordycipitaceae): introducing two new species and one new record from China
- Two new acremonium-like species, Paragibellulopsis sinensis sp. nov. and Phialoparvum sinense sp. nov. (Sordariomycetes, Ascomycota) from China
- Two new species of Neohelicosporium (Tubeufiaceae, Tubeufiales) from freshwater and terrestrial habitats in China
- Phylogenetic evidence reveal a close relationship between Amphichorda and Ovicillium in Bionectriaceae (Hypocreales)
- Introducing Muciflexus inthanonensis gen. et sp. nov. and updates on Ochronectria (Hypocreales): New insights from leaf litter fungi in Doi Inthanon National Park, Northern Thailand
- Harposporium incensis sp. nov. a South American cordycipitoid species exhibiting inter-phylum host-jumping and having potential as a biological control agent for pest management
- Morphological and molecular analyses reveal two new species of Gibellula (Cordycipitaceae, Hypocreales) from China
- A Loop-Mediated Isothermal Amplification Portable Assay for Visual Detection of Phacidiopycnis washingtonensis and Phacidiopycnis pyri on Pome Fruit
- Molecular phylogeny and morphology reveal two new species of Pleurocordyceps luopingensis sp. nov. (Polycephalomycetaceae) and Pseudometarhizium cangyuanense sp. nov. (Clavicipitaceae) from Yunnan, southwestern China
- Propiconazole Sensitivity of Geotrichum citri-aurantii and Penicillium digitatum Isolates in the Western and Eastern Cape Provinces of South Africa
- Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: Potential for conservation biological control
- Morphological and phylogenetic analyses reveal two new species of Rhodoveronaea (Rhamphoriaceae, Rhamphoriales) from China
- Family matters inside the order Agaricales: systematic reorganization and classification of incertae sedis clitocyboid, pleurotoid and tricholomatoid taxa based on an updated 6-gene phylogeny
- Two new species of Neohelicomyces (Tubeufiaceae, Tubeufiales) from Hainan Province, China
- Expanding the genus Neobaryopsis (Calcarisporiaceae, Hypocreales): descriptions of two new species and two new combinations based on morphological and molecular data
- Morphological and phylogenetic analyses reveal two new species of Camporesiomyces (Tubeufiaceae, Tubeufiales) from terrestrial habitats in China
- Phylogeny of Phyllozyma (Spiculogloeaceae, Spiculogloeales) with description of four new species from China
- Ganoderma adspersum, the Cause of Extensive Wood Decay of Almond Trees in California Orchards
- Unveiling species diversity within early-diverging fungi from China XVI: Five new Absidia and a new Gongronella species (Cunninghamellaceae, Mucoromycota)
- Evolution of lifestyles in Capnodiales
- Identification and characterization of fungi associated with leaf spot/blight and melting-out of turfgrass in Algeria
- Catalogue of fungi in China 1. New taxa of plant-inhabiting fungi
- Multi-gene phylogeny and morphological characters reveal seven new species of Micropsalliota (Agaricales, Agaricaceae) from southern China, with an updated key for the species distributed in China
- Diversity of culturable fungi in six Tibetan Plateau lakes, with descriptions of eight new taxa
- Morphology and multigene phylogeny revealed a new species and a new record of Conocybe (Bolbitiaceae) from Oman
- Phylogeny of tremellomycetous yeasts and related dimorphic and filamentous basidiomycetes reconstructed from multiple gene sequence analyses
- Revision ofCerinomyces(Dacrymycetes,Basidiomycota) with notes on morphologically and historically related taxa
- Morphological and phylogenetic analyses reveal four novel species of Distoseptispora (Distoseptisporaceae, Distoseptisporales) from southern China
- Taxonomic reevaluation on <i>Pyrrhoderma</i> (<i>Basidiomycota</i>, <i>Hymenochaetales</i>) and reclassification of <i>Pyrrhoderma</i> species based on phylogenetic and type studies
- <i>Anthostomella</i>-like fungi on bamboo: four new genera belonging to a new family <i>Pallidoperidiaceae</i> (<i>Xylariales</i>)
- Taxonomic study of Endogonaceae in the Japanese islands: New species ofEndogone, Jimgerdemannia, andVinositunica, gen. nov.
- Detection of Delphinella shoot blight in plantations of balsam fir (Abies balsamea) Christmas trees in Quebec, Canada
- Revision of leccinoid fungi, with emphasis on North American taxa, based on molecular and morphological data
- Kaziboletus, a new boletoid genus of Boletaceae associated with Shorea robusta in Bangladesh
- Phylogenetic overview of the Boletineae
- On some forgotten species of Exidia and Myxarium (Auriculariales, Basidiomycota)
- Basic and Applied Research on Entomopathogenic Fungi
- Fungal Diversity Revisited: 2.2 to 3.8 Million Species
- Diagnostics for a troubled backbone: testing topological hypotheses of trapelioid lichenized fungi in a large-scale phylogeny of Ostropomycetidae (Lecanoromycetes)
- Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. [europepmc]
- Evolution and ecophysiology of the industrial producer Hypocrea jecorina (Anamorph Trichoderma reesei) and a new sympatric agamospecies related to it. [europepmc]
- Phylogenetic and biogeographic implications inferred by mitochondrial intergenic region analyses and ITS1-5.8S-ITS2 of the entomopathogenic fungi Beauveria bassiana and B. brongniartii. [europepmc]
- The dawn of symbiosis between plants and fungi. [europepmc]
- Interspecific sex in grass smuts and the genetic diversity of their pheromone-receptor system. [europepmc]
- Multi-locus phylogeny of lethal amanitas: implications for species diversity and historical biogeography. [europepmc]
- Take-all or nothing. [europepmc]
- Comparative genomics of Beauveria bassiana: uncovering signatures of virulence against mosquitoes. [europepmc]
- Resolved phylogeny and biogeography of the root pathogen Armillaria and its gasteroid relative, Guyanagaster. [europepmc]
- Evolution of ectomycorrhizas as a driver of diversification and biogeographic patterns in the model mycorrhizal mushroom genus Laccaria. [europepmc]
- Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community. [europepmc]
- Fungal Diversity Revisited: 2.2 to 3.8 Million Species. [europepmc]
- Culturable mycobiota from Karst caves in China, with descriptions of 20 new species. [europepmc]
- Morphological and molecular identification of two new Ganoderma species on Casuarina equisetifolia from China. [europepmc]
- Inside Plectosphaerellaceae . [europepmc]
- Malassezia vespertilionis sp. nov.: a new cold-tolerant species of yeast isolated from bats. [europepmc]
- Multi-locus phylogeny and taxonomy of Exserohilum . [europepmc]
- Dual DNA Barcoding for the Molecular Identification of the Agents of Invasive Fungal Infections. [europepmc]
- Taxonomy and phylogeny of Postia. Multi-gene phylogeny and taxonomy of the brown-rot fungi: Postia ( Polyporales , Basidiomycota ) and related genera. [europepmc]
- Revisiting Metarhizium and the description of new species from Thailand. [europepmc]
- Evolution of lifestyles in Capnodiales . [europepmc]
- Multi-gene phylogeny and taxonomy of Amauroderma s.lat. ( Ganodermataceae ). [europepmc]
- Phylogenetic delimitation of Apiospora and Arthrinium . [europepmc]
- Fungal Planet description sheets: 1112-1181. [europepmc]
- Species diversity, systematic revision and molecular phylogeny of Ganodermataceae ( Polyporales , Basidiomycota ) with an emphasis on Chinese collections. [europepmc]
Related