The use of DAPI for identifying and counting aquatic microflora1
1980/09/01 by Karen G. Porter, Yvette S. Feig · 5,090 citations
Biochemistry, Genetics and Molecular Biology · Chemistry · Environmental Science · #Acridine orange #Algae #Biology #Botany #Chemistry #Chromatography #DAPI #Identification and Quantification in Food #Microbial Community Ecology and Physiology #Molecular Biology Techniques and Applications #Staining
paper · pdf · doi:10.4319/lo.1980.25.5.0943
published in Limnology and Oceanography 25(5), 943-948 (Wiley)
openalex publication_date 1980/09/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/05
Abstract
A highly specific and sensitive fluorescing DNA stain, 4′6‐diamidino‐2‐phenylindole (DAPI) was compared with acridine orange (AO) for counting aquatic microflora. Use of DAPI improved visualization and counting of <1‐ µ m bacteria and blue‐green algae in seston‐rich samples and extended sample storage to at least 24 weeks.
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