2021/04/09 by Sook Kyung Shin, Yeonhui Lee, Hayoung Kwon +3 · 22 citations
Agricultural and Biological Sciences · Chemistry · Earth and Planetary Sciences · Environmental Science · #Algae #Biochemistry #Biology #Boiling #Botany #Chemistry #Chromatography #DNA #DNA extraction #Extraction (chemistry) #Gene #Marine Bivalve and Aquaculture Studies #Marine and coastal plant biology #Polymerase chain reaction #Seaweed-derived Bioactive Compounds #genomic DNA
paper · doi:10.1111/jpy.13175
published in Journal of Phycology 57(4), 1368-1372 (Wiley)
openalex publication_date 2021/04/09 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/28
A rapid, simple, and cost-effective total DNA extraction method referred to as a direct boiling method for PCR-based algal species determination was validated using representative species of green, brown, and red algae. Each sample was briefly minced in two buffers, Tris-EDTA (TE) or PCR buffer, and transferred to a heat block for boiling at 98°C for 5 min. No detergent was used in this experiment. The entire DNA isolation procedure was completed within 10 min. After brief centrifugation, the supernatant was directly used as a template for PCR. As a result, all genomic DNA markers were successfully amplified and sequenced from each algal taxon. Regardless of DNA quality, the direct boiling method is very suitable to identify unknown species of algae from a large amount of samples in a limited time and can be applied broadly to routine seasonal or annual monitoring of algae. DNA from an Undaria pinnatifida sporophyte, however, was not successfully extracted by this direct boiling method, probably due to a high concentration of polysaccharides.