CD34⁺Hematopoietic Progenitor Cell Subsets Exhibit Differential Ability To Maintain Human Cytomegalovirus Latency and Persistence

2020/11/10 by Lindsey B. Crawford, Meaghan H. Hancock, Hillary M. Struthers +5 · 2 citations
Medicine · Immunology and Microbiology · #Cytomegalovirus and herpesvirus research #Immune Cell Function and Interaction #Hematopoietic Stem Cell Transplantation

paper · doi:10.1128/jvi.02105-20

Abstract

hematopoietic progenitor cells (HPCs) are a critical viral reservoir; however, these cells are a heterogeneous pool with donor-to-donor variation in functional, genetic, and phenotypic characteristics. We generated a novel system using embryonic stem cell lines to model HCMV latency and reactivation in HPCs with a consistent cellular background. Our study defined three key stem cell subsets with differentially regulated latent and replicative states, which provide cellular candidates for isolation and treatment of transplant-mediated disease. This work provides a direction toward developing strategies to control the switch between latency and reactivation.

Citations

• Human Cytomegalovirus Stimulates Monocyte-to-Macrophage Differentiation via the Temporal Regulation of Caspase 3
• ELDA: Extreme limiting dilution analysis for comparing depleted and enriched populations in stem cell and other assays
• Human Cytomegalovirus Induces Monocyte Differentiation and Migration as a Strategy for Dissemination and Persistence
• Reactivation of Latent Human Cytomegalovirus in CD14⁺Monocytes Is Differentiation Dependent

Cited by

• Human cytomegalovirus modulates mTORC1 to redirect mRNA translation within quiescently infected monocytes
• Cytomegalovirus latency—the sum of subtleties

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