Long-Term Lymphohematopoietic Reconstitution by a Single CD34-Low/Negative Hematopoietic Stem Cell

1996/07/12 by Masatake Osawa, Ken-ichi Hanada, Ken‐ichi Hanada +2 · 31 citations
Medicine · Biochemistry, Genetics and Molecular Biology · #Hematopoietic Stem Cell Transplantation #Zebrafish Biomedical Research Applications #Pluripotent Stem Cells Research

paper · doi:10.1126/science.273.5272.242

Abstract

Hematopoietic stem cells (HSCs) supply all blood cells throughout life by making use of their self-renewal and multilineage differentiation capabilities. A monoclonal antibody raised to the mouse homolog of CD34 (mCD34) was used to purify mouse HSCs to near homogeneity. Unlike in humans, primitive adult mouse bone marrow HSCs were detected in the mCD34 low to negative fraction. Injection of a single mCD34(lo/-), c-Kit+, Sca-1(+), lineage markers negative (Lin-) cell resulted in long-term reconstitution of the lymphohematopoietic system in 21 percent of recipients. Thus, the purified HSC population should enable analysis of the self-renewal and multilineage differentiation of individual HSCs.

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