Experimental Transmission of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) Among White-Tailed Deer by Amblyomma americanum (Acari: Ixodidae)

1995/05/01 by S. A. Ewing, Jacqueline E. Dawson, J. E. Dawson +11 · 27 citations
Agricultural and Biological Sciences · Immunology and Microbiology · Veterinary · #Acari #Amblyomma americanum #Biology #Ehrlichia #Ehrlichia chaffeensis #Ehrlichiosis #Helminth infection and control #Ixodes scapularis #Ixodidae #Odocoileus #Rickettsiales #Study of Mite Species #Tick #Tick-borne disease #Vector-borne infectious diseases #Virology #Zoology

paper · doi:10.1093/jmedent/32.3.368

openalex publication_date 1995/05/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/07/29

Abstract

Ehrlichia chaffeensis Anderson, Dawson & Wilson, causative agent of human (predominantly monocytic) ehrlichiosis, was successfully transmitted experimentally by Amblyomma americanum (L.) to white-tailed deer, Odocoileus virginianus (Zimmerman). Deer were needle-exposed intravenously to E. chaffeensis in tissue-culture canine macrophage (DH82) cells, and 11 d later were exposed to laboratory-reared A. americanum larvae, nymphs, and adults for acquisition feeding. Three months after this feeding, naive deer and dogs were exposed to recently molted nymphs and adults. Attempted reisolation of the pathogen by way of tissue culture was successful from one needle-exposed deer but not from the tick-exposed deer or dogs. Based on serologic evidence and polymerase chain reaction data, both nymphal and adult ticks transmitted E. chaffeensis to naive deer but not to dogs.

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• Detection of Ehrlichia spp. in the blood of wild white-tailed deer in Missouri by PCR assay and serologic analysis. [europepmc]
• Isolation of an Anaplasma sp. organism from white-tailed deer by tick cell culture. [europepmc]
• Development of a p28-based PCR assay for Ehrlichia chaffeensis. [europepmc]
• Transstadial and intrastadial experimental transmission of Ehrlichia canis by male Rhipicephalus sanguineus. [europepmc]
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• Tick-Borne Zoonoses in the United States: Persistent and Emerging Threats to Human Health. [europepmc]
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