Experimental Infection of Amblyomma americanum (Acari: Ixodidae) With Bourbon Virus (Orthomyxoviridae: Thogotovirus)
2020/10/09 by Marvin S Godsey, Marvin S. Godsey, Dominic Rose +10 · 23 citations
Immunology and Microbiology · Medicine · #Acari #Amblyomma americanum #Biology #Enzootic #Ixodidae #Microbiology #Mosquito-borne diseases and control #Tick #Vector-borne infectious diseases #Viral Infections and Vectors #Virology #Virus #Zoology
paper · pdf · doi:10.1093/jme/tjaa191
openalex publication_date 2020/10/09 · openalex created_date 2020/10/15 · openalex updated_date 2026/07/29
Abstract
Following the recent discovery of Bourbon virus (BRBV) as a human pathogen, and the isolation of the virus from Amblyomma americanum (L.) collected near the location of a fatal human case, we undertook a series of experiments to assess the laboratory vector competence of this tick species for BRBV. Larval ticks were infected using an immersion technique, and transstadial transmission of virus to the nymphal and then to the adult stages was demonstrated. Transstadially infected nymphs transmitted virus to adult ticks at very high rates during cofeeding, indicating the presence of infectious virus in the saliva of engorging ticks. Vertical transmission by transstadially infected females to their progeny occurred, but at a low rate. Rabbits fed on by infected ticks of all active life stages developed high titers of antibody to the virus, demonstrating host exposure to BRBV antigens/live virus during tick blood feeding. These results demonstrate that A. americanum is a competent vector of BRBV and indicate that cofeeding could be critical for enzootic maintenance.
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