Direct detection of DNA methylation during single-molecule, real-time sequencing

2010/05/09 by Benjamin A. Flusberg, Benjamin A Flusberg, Dale R. Webster +12 · 1,353 citations
Biochemistry, Genetics and Molecular Biology · #5-Methylcytosine #Base pair #Base sequence #Biology #Bisulfite #Bisulfite sequencing #Computational biology #DNA #DNA methylation #DNA nanoball sequencing #DNA sequencer #DNA sequencing #Epigenetics and DNA Methylation #Gene #Gene expression #Genetic Syndromes and Imprinting #Genetics #Genomic library #Illumina Methylation Assay #Methylated DNA immunoprecipitation #Methylation #Molecular biology #Polymerase #RNA modifications and cancer #Sequencing by ligation #Single molecule real time sequencing

paper · open access · doi:10.1038/nmeth.1459

published in Nature Methods 7(6), 461-465 (Springer Science and Business Media LLC)

crossref issued 2010/05/09 · crossref published 2010/05/09 · crossref published-online 2010/05/09 · openalex publication_date 2010/05/09 · crossref created 2010/05/09 · crossref published-print 2010/06/01 · crossref deposited 2023/05/18 · openalex created_date 2025/10/10 · crossref indexed 2026/08/08 · openalex updated_date 2026/08/08

Citations

• Epigenetic regulation of gene expression: how the genome integrates intrinsic and environmental signals
• Selective aluminum passivation for targeted immobilization of single DNA polymerase molecules in zero-mode waveguide nanostructures
• Continuous base identification for single-molecule nanopore DNA sequencing
• A genome-wide analysis of CpG dinucleotides in the human genome distinguishes two distinct classes of promoters

Cited by

• Detecting DNA cytosine methylation using nanopore sequencing
• Genome-wide mapping of methylated adenine residues in pathogenic Escherichia coli using single-molecule real-time sequencing
• Entering the era of bacterial epigenomics with single molecule real time DNA sequencing
• Detection and mapping of 5-methylcytosine and 5-hydroxymethylcytosine with nanopore MspA
• Modeling kinetic rate variation in third generation DNA sequencing data to detect putative modifications to DNA bases
• Comprehensive Methylome Characterization of Mycoplasma genitalium and Mycoplasma pneumoniae at Single-Base Resolution
• Extensive N4 cytosine methylation is essential for Marchantia sperm function
• Epigenomics [wikipedia]