1992/01/01 by Edward Perez‐Reyes, Antonio Castellano, Hyun Sil Kim +5 · 411 citations
Agricultural and Biological Sciences · Biochemistry, Genetics and Molecular Biology · Chemistry · Medicine · #BETA (programming language) #Biochemistry #Biology #Calcium #Calcium channel #Cardiac electrophysiology and arrhythmias #Cell biology #Chemistry #Cloning (programming) #Computer science #Gene #Internal medicine #Ion channel regulation and function #L-type calcium channel #Medicine #Molecular biology #Pharmacological Effects and Assays #Protein subunit
paper · doi:10.1016/s0021-9258(18)46015-2
published in Journal of Biological Chemistry 267(3), 1792-1797 (Elsevier BV)
openalex publication_date 1992/01/01 · openalex created_date 2025/10/10 · openalex updated_date 2026/08/01
The skeletal muscle dihydropyridine receptor/Ca2+ channel is composed of five protein components (alpha 1, alpha 2 delta, beta, and gamma). Only two such components, alpha 1 and alpha 2, have been identified in heart. The present study reports the cloning and expression of a novel beta gene that is expressed in heart, lung, and brain. Coexpression of this beta with a cardiac alpha 1 in Xenopus oocytes causes the following changes in Ca2+ channel activity: it increases peak currents, accelerates activation kinetics, and shifts the current-voltage relationship toward more hyperpolarized potentials. It also increases dihydropyridine binding to alpha 1 in COS cells. These results indicate that the cardiac L-type Ca2+ channel has a similar subunit structure as in skeletal muscle, and provides evidence for the modulatory role of the beta subunit.