Standardized protocols for differentiation of THP-1 cells to macrophages with distinct M(IFNγ+LPS), M(IL-4) and M(IL-10) phenotypes

2020/02/04 by Euan W. Baxter, E.W. Baxter, A.E. Graham +11 · 28 citations
Immunology and Microbiology · #Immune cells in cancer #Immune Cell Function and Interaction #Immunotherapy and Immune Responses

paper · doi:10.1016/j.jim.2019.112721

Cited by

• Selection and validation of reference genes for qPCR analysis of differentiation and maturation of THP‐1 cells into M1 macrophage‐like cells
• High-throughput bioprinted 3D cultures for probing host–pathogen interactions in bioinspired microenvironments
• Signals from the sea: the structural peculiarity of lipid A and weak immunostimulatory lipopolysaccharide from Rheinheimera japonica
• Tumor-on-a-chip platform to interrogate the role of macrophages in tumor progression. [europepmc]
• Secreted Factors From Proinflammatory Macrophages Promote an Osteoblast-Like Phenotype in Valvular Interstitial Cells. [europepmc]
• Microfluidic model of monocyte extravasation reveals the role of hemodynamics and subendothelial matrix mechanics in regulating endothelial integrity. [europepmc]
• Understanding immune-modulatory efficacy in vitro. [europepmc]
• An Apple a Day Keeps the Doctor Away: Potential Role of miRNA 146 on Macrophages Treated with Exosomes Derived from Apples. [europepmc]
• Increased Levels of Interleukin-36 in Obesity and Type 2 Diabetes Fuel Adipose Tissue Inflammation by Inducing Its Own Expression and Release by Adipocytes and Macrophages. [europepmc]
• A multifunctional micropore-forming bioink with enhanced anti-bacterial and anti-inflammatory properties. [europepmc]
• The Acute Toxicity of Mineral Fibres: A Systematic In Vitro Study Using Different THP-1 Macrophage Phenotypes. [europepmc]
• Dihydronicotinamide Riboside Is a Potent NAD + Precursor Promoting a Pro-Inflammatory Phenotype in Macrophages. [europepmc]
• Cyclic Mechanical Stresses Alter Erythrocyte Membrane Composition and Microstructure and Trigger Macrophage Phagocytosis. [europepmc]
• Dealing with Macrophage Plasticity to Address Therapeutic Challenges in Head and Neck Cancers. [europepmc]
• Vascular restenosis reduction with platelet membrane coated nanoparticle directed M2 macrophage polarization. [europepmc]
• Comparison of monocytic cell lines U937 and THP-1 as macrophage models for in vitro studies. [europepmc]
• Resting time after phorbol 12-myristate 13-acetate in THP-1 derived macrophages provides a non-biased model for the study of NLRP3 inflammasome. [europepmc]
• Therapeutic Potential of Bioactive Components from Scutellaria baicalensis Georgi in Inflammatory Bowel Disease and Colorectal Cancer: A Review. [europepmc]
• Transcriptomic Analysis of Macrophage Polarization Protocols: Vitamin D 3 or IL-4 and IL-13 Do Not Polarize THP-1 Monocytes into Reliable M2 Macrophages. [europepmc]
• Tumor-Associated Macrophage Subsets: Shaping Polarization and Targeting. [europepmc]
• Spatial imaging of glycoRNA in single cells with ARPLA. [europepmc]
• Induced Pluripotent Stem Cell-Derived Extracellular Vesicles Promote Wound Repair in a Diabetic Mouse Model via an Anti-Inflammatory Immunomodulatory Mechanism. [europepmc]
• Identification of driver genes in lupus nephritis based on comprehensive bioinformatics and machine learning. [europepmc]
• The interaction between DNA methylation and tumor immune microenvironment: from the laboratory to clinical applications. [europepmc]
• Axl and MerTK regulate synovial inflammation and are modulated by IL-6 inhibition in rheumatoid arthritis. [europepmc]
• Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype. [europepmc]
• Dual Role of microRNA-146a in Experimental Inflammation in Human Pulmonary Epithelial and Immune Cells and Expression in Inflammatory Lung Diseases. [europepmc]
• Glycerophospholipid remodeling is critical for orthoflavivirus infection. [europepmc]